Dataset Information

SMAX1/SMXL2 interacting proteins in Arabidopsis seedling through IP-MS

ABSTRACT: Karrikins (KARs) are a class of butenolide molecules found in smoke. KARs regulate many aspects of plant development and environmental responses, including Seed germination, hypocotyl elongation, leaf shape, root development, and resistance to drought, salt, and heat stress. The transcriptional repressor proteins SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-like2 (SMXL2) integrate karrikin signaling by repressing gene expression through direct binding to promoters or through inhibiting activities of transcription factors. To elucidate the mechanism by which SMAX1 and SMXL2 mediate KAR signaling, we performed immunoprecipitation followed by mass spectrometry (IP-MS) analysis and identified peptides in transgenic seedlings that overexpress GFP, SMAX1-GFP, and SMXL2-GFP, respectively.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root, Leaf

SUBMITTER: wenwen chang

LAB HEAD: Lei Wang

PROVIDER: PXD052400 | Pride | 2024-06-22

REPOSITORIES: Pride

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Publications

Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis.

Chang Wenwen W Qiao Qiao Q Li Qingtian Q Li Xin X Li Yanyan Y Huang Xiahe X Wang Yingchun Y Li Jiayang J Wang Bing B Wang Lei L

Molecular plant 20240527 7

Karrikins and strigolactones govern plant development and environmental responses through closely related signaling pathways. The transcriptional repressor proteins SUPPRESSOR OF MAX2 1 (SMAX1), SMAX1-like2 (SMXL2), and D53-like SMXLs mediate karrikin and strigolactone signaling by directly binding downstream genes or by inhibiting the activities of transcription factors. In this study, we characterized the non-transcriptional regulatory activities of SMXL proteins in Arabidopsis. We discovered ...[more]

PMID: 38807366

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Project description:In response to environmental light signals, transcriptomic adjustment plays an important role in Arabidopsis seed germination and seedling development. G-box cis-element is commonly present in promoters of genes positively or negatively responding to the light signal. For the pursuit of additional transcriptional regulator modulating light-mediated transcriptome changes, we have identified AtbZIP16, a basic region/leucine zipper motif transcription factor, via G-box DNA affinity chromatography. We have confirmed that AtbZIP16 possesses G-box-specific binding activity. Analyses of atbzip16 mutants indicate that AtbZIP16 is a negative regulator in phyB-mediated inhibition of cell elongation, but a positive regulator in phytochrome-mediated seed germination process. Transcriptomic analysis supports that AtbZIP16 is primarily a transcriptional repressor regulating light-, GA- and ABA-responsive genes. Chromatin immunoprecipitation study revealed that AtbZIP16 could directly target RGL2, a DELLA gene, and indirectly repress the expression of PIL5 gene, which encodes a bHLH protein inhibiting seed germination in Arabidopsis. Our study indicated that, through repressing the expression of RGL2 and the antagonizing the expression of PIL5, AtbZIP16 functions to promote seed germination and hypocotyl elongation during early stages of Arabidopsis seedling development. In response to environmental light signals, transcriptomic adjustment plays an important role in Arabidopsis seed germination and seedling development. G-box cis-element is commonly present in promoters of genes positively or negatively responding to the light signal. For the pursuit of additional transcriptional regulator modulating light-mediated transcriptome changes, we have identified AtbZIP16, a basic region/leucine zipper motif transcription factor, via G-box DNA affinity chromatography. We have confirmed that AtbZIP16 possesses G-box-specific binding activity. Analyses of atbzip16 mutants indicate that AtbZIP16 is a negative regulator in phyB-mediated inhibition of cell elongation, but a positive regulator in phytochrome-mediated seed germination process. Transcriptomic analysis supports that AtbZIP16 is primarily a transcriptional repressor regulating light-, GA- and ABA-responsive genes. Chromatin immunoprecipitation study revealed that AtbZIP16 could directly target RGL2, a DELLA gene, and indirectly repress the expression of PIL5 gene, which encodes a bHLH protein inhibiting seed germination in Arabidopsis. Our study indicated that, through repressing the expression of RGL2 and the antagonizing the expression of PIL5, AtbZIP16 functions to promote seed germination and hypocotyl elongation during early stages of Arabidopsis seedling development. Three biological replicates for 4-d-old seedlings grown under dark or red-light and long-day (0.5 ?mole m-2 sec-1) contitions.

			Action	DRS
	GFP.raw	Raw
	GFP.txt	Txt
	SMAX1-GFP.raw	Raw
	SMAX1-GFP.txt	Txt
	SMXL2-GFP.raw	Raw