Dataset Information

Proteomic study of localized tissue necrosis by Naja atra venom

ABSTRACT: Naja atra bites frequently cause immediate and serious disease. N. atra venom contains a complex of toxic components that can cause immediate and direct harm to the patient's skin tissue. If not treated effectively, it can readily progress to widespread localized necrosis, which in severe cases may result in impairment or amputation. However, the underlying causes for this localized tissue necrosis remain unknown. This study aims to investigate the protein basis of N. atra venom -induced localized tissue necrosis at the whole organism and dynamic network levels, as well as to understand the pathogenic process of this necrosis from a molecular standpoint. Venom was injected into Bama miniature pigs to induce ulcers, and exudate samples from the wounds were collected at different points over time. This model aims to provide a means to observe the ulceration process and collect exudate for further analysis regarding the effects of the venom. Through label-free proteomics analysis, we identified all protein components present in the wound exudate. The proteomics results revealed that 1119, 1016, 938, 864, and 855 proteins were identified in the exudate at 6 hours, 12 hours, 24 hours, 36 hours, and 48 hours after snake venom injection, respectively, and a total of 431 differentially expressed proteins were screened from the proteins in the exudate at the five time periods. Bioinformatics and Parallel Reaction Monitoring (PRM) with Enzyme-linked immunosorbent assay (ELISA)were used to validate further screening, and we eventually identified S100A8, MMP-2, MIF, and IDH2 as proteins associated with local tissue necrosis. In this study, we established a Bama miniature pig model for N. atra venom injection, and performed proteomic analysis of the wound exudate, which provides important insights into the molecular pathology of snakebite-induced tissue necrosis and potential theoretical bases for clinical treatment.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Wound Fluid

SUBMITTER: ManQi Xiao

LAB HEAD: Ming Liao

PROVIDER: PXD052498 | Pride | 2024-07-03

REPOSITORIES: Pride

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			Action	DRS
	P20220905847_foeh_1.raw	Raw
	P20220905847_foeh_2.raw	Raw
	P20220905847_foeh_3.raw	Raw
	P20220905847_six_1_20221103190518.raw	Raw
	P20220905847_six_2.raw	Raw

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Proteomic study of localized tissue necrosis by Naja atra venom.

Guan Zhezhe Z Xiao Manqi M Hu Shaocong S Li Yalan Y Mo Caifeng C Yin Yalong Y Li Ruopeng R Zhang Ziyan Z Zhang Xuerong X Liao Ming M

Toxicon : official journal of the International Society on Toxinology 20240625

Naja atra bites often result in immediate and severe illness. The venom of N. atra contains a complex mixture of toxins that can cause significant damage to the patient's skin tissue. If left untreated, this condition can progress to localized necrosis, potentially resulting in impairment or even amputation in severe cases. Despite the known effects of the venom, the exact mechanisms underlying this tissue necrosis are not fully understood. This study aimed to investigate the protein components ...[more]

PMID: 38925341

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