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Proteomic analysis of Tmem232-/- and wild type mice sperm

ABSTRACT: The sperm were harvested from caudal epididymides of 8-week-old Tmem232-/- and wild-type adult male mice, and subjected to LC-MS/MS analysis.The total proteins of 5,199 were identified and 4,380 of them were quantified. Results showed that 385 differentially expressed proteins were identified including 88 up-regulated and 255 down-regulated genes (Fold Change≥1.5, P<0.05), and group comparisons were presented in a volcano plot.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cauda Epididymis, Sperm

DISEASE(S): Male Infertility

SUBMITTER: cai xinying

LAB HEAD: Fengsong Wang

PROVIDER: PXD052758 | Pride | 2024-11-15

REPOSITORIES: pride

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Publications

Solid-phase Mannich condensation of amines, aldehydes, and alkynes: investigation of diverse aldehyde inputs.

Youngman M A MA Dax S L SL

Journal of combinatorial chemistry 20010901 5

Terminal alkynes, secondary amines, and aldehydes undergo "solid-phase Mannich condensation". A set of diverse aldehyde inputs was examined. Aliphatic, aralkyl, aryl, and heteroaryl carboxaldehydes give good yields of Mannich adduct of high purity. Benzaldehydes containing electron-donating substituents that decrease the electrophilicity of the carbonyl center, or heteroaryl aldehydes that are similarly deactivated by resonance effects, do not undergo reaction. ...[more]

PMID: 11549365

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