Proteomics

Dataset Information

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Redox-proteomic analysis of pancreatic β-cells upon glucose stimulation

ABSTRACT: Redox signaling mediated by reversible oxidative cysteine thiol modifications is crucial for driving cellular adaptation to dynamic environmental changes, maintaining homeostasis, and ensuring proper function. This is particularly critical in pancreatic β-cells, which are highly metabolically active and play a specialized role in whole organism glucose homeostasis. Glucose stimulation in β-cells triggers signals leading to insulin secretion, including changes in ATP/ADP ratio and intracellular calcium levels. Additionally, lipid metabolism and reactive oxygen species (ROS) signaling are essential for β-cell function and health. We employed IodoTMT isobaric labeling combined with tandem mass spectrometry to elucidate redox signaling pathways in pancreatic β-cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Barbora Salovska  

LAB HEAD: Blanka Holendova

PROVIDER: PXD052765 | Pride | 2024-09-30

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
655-2001.raw Raw
655-2002.raw Raw
File_description.xlsx Xlsx
PLK050_EXPL_210816_iodoTMT_mix1_4uL_DDA_150M_120G.raw Raw
PLK050_EXPL_210816_iodoTMT_mix2_4uL_DDA_150M_120G.raw Raw
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Publications

Beyond glucose: The crucial role of redox signaling in β-cell metabolic adaptation.

Holendová Blanka B   Šalovská Barbora B   Benáková Štěpánka Š   Plecitá-Hlavatá Lydie L  

Metabolism: clinical and experimental 20240912

<h4>Objective</h4>Redox signaling mediated by reversible oxidative cysteine thiol modifications is crucial for driving cellular adaptation to dynamic environmental changes, maintaining homeostasis, and ensuring proper function. This is particularly critical in pancreatic β-cells, which are highly metabolically active and play a specialized role in whole organism glucose homeostasis. Glucose stimulation in β-cells triggers signals leading to insulin secretion, including changes in ATP/ADP ratio a  ...[more]

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