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Mycobacterium tuberculosis surface proteomics

ABSTRACT: Surface shaving Mtb using two concentrations of trypsin: 0.4 ug/mL and 0.05 ug/mL. Both bacteria and supernatant without the bacteria were subject to trypsinization, to allow for a ratio between samples to identify candidate surface proteins.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mycobacterium Tuberculosis H37rv

SUBMITTER: Bianca Lepe

LAB HEAD: Bryan D. Bryson

PROVIDER: PXD052936 | Pride | 2024-12-11

REPOSITORIES: pride

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			Action	DRS
	1162_BL_6plex_01.mzML	Mzml
	1162_BL_6plex_01.mzid.gz	Mzid
	1300_BL_6plex_01.mzML	Mzml
	1300_BL_6plex_01.mzid.gz	Mzid
	checksum.txt	Txt

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Publications

Protease shaving of Mycobacterium tuberculosis facilitates vaccine antigen discovery and delivery of novel cargoes to the Mtb surface.

Lepe Bianca A BA Zheng Christine R CR Leddy Owen K OK Allsup Benjamin L BL Solomon Sydney L SL Bryson Bryan D BD

bioRxiv : the preprint server for biology 20240702

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is the leading cause of infectious disease death and lacks a vaccine capable of protecting adults from pulmonary TB. Studies have shown that Mtb uses a variety of mechanisms to evade host immunity. Secreted Mtb proteins such as Type VII secretion system substrates have been characterized for their ability to modulate anti-Mtb immunity; however, studies of other pathogens such as Salmonella Typhi and Staphylococcus au ...[more]

PMID: 39005324

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