Proteomics

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Matrisome Proteomics Reveals Novel Mediators of Muscle Remodeling with Aerobic Exercise Training


ABSTRACT: Skeletal muscle has a unique ability to remodel in response to stimuli such as contraction and aerobic exercise training. Phenotypic changes in muscle that occur with training such as a switch to a more oxidative fiber type, and increased capillary density contribute to the well-known health benefits of aerobic exercise. The muscle matrisome likely plays an important role in muscle remodeling with exercise. However, due to technical limitations in studying muscle ECM proteins, which are highly insoluble, little is known about the muscle matrisome and how it contributes to muscle remodeling. Here, we utilized two-fraction methodology to extract muscle proteins, combined with multiplexed tandem mass tag proteomic technology to identify 161 unique ECM proteins in mouse skeletal muscle. In addition, we demonstrate that aerobic exercise training induces remodeling of a significant proportion of the muscle matrisome. We performed follow-up experiments to validate exercise-regulated ECM targets in a separate cohort of mice using Western blotting and immunofluorescence imaging. Our data demonstrate that changes in several key ECM targets are strongly associated with muscle remodeling processes such as increased capillary density in mice. We also identify LOXL1 as a novel muscle ECM target associated with aerobic capacity in humans. In addition, publically available data and databases were used for in silico modeling to determine the likely cellular sources of exercise-induced ECM remodeling targets and identify ECM interaction networks. This work greatly enhances our understanding of ECM content and function in skeletal muscle and demonstrates an important role for ECM remodeling in the adaptive response to exercise.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Joao Paulo  

LAB HEAD: Sarah J. Lessard

PROVIDER: PXD053003 | Pride | 2024-07-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
JM7038_tcmp-848_F1.raw Raw
JM7038_tcmp_848_F1_calMS1.mzIdentML Mzid
JM7039_tcmp-848_F2.raw Raw
JM7039_tcmp_848_F2_calMS1.mzIdentML Mzid
JM7040_tcmp-848_F3.raw Raw
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