Proteomics

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High-throughput proteomic and phosphoproteomic approaches revealing the salt-responsive mechanisms in two different rice varieties


ABSTRACT: Salinity stress induces ionic and osmotic imbalances in rice plants that in turn negatively affect photosynthesis rate, resulting in growth retardation and yield penalty. Efforts have, therefore, been carried out to understand the mechanism of salt tolerance, however, the complexity of biological processes at proteome levels remains a major challenge. Here, we performed a comparative proteome and phosphoproteome profiling of microsome enriched fractions of salt-tolerant (cv. IR73; indica) and salt-susceptible (cv. Donjin; japonica) rice varieties. This approach led to the identification of 5,856 proteins, of which 473 and 484 proteins showed differential modulation between DJ and IR sample sets, respectively. The phosphoproteome analysis led to the identification of a total 10,873 phosphopeptides of these 2,929 and 3,049 significant phosphopeptides were identified in DJ and IR sample sets, respectively. The integration of proteome and phosphoproteome data showed activation of ABA and Ca2+ signaling components exclusively in IR in response to salt stress. Taken together, our results highlight the changes at proteome and phosphoproteome levels and provide a mechanistic understand of salt stress tolerance in rice.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa Indica Group X Oryza Sativa Japonica Group

TISSUE(S): Leaf

DISEASE(S): Salt

SUBMITTER: Sun Tae Kim  

LAB HEAD: Sun Tae Kim

PROVIDER: PXD053678 | Pride | 2024-12-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BNU_A1_01.raw Raw
BNU_A1_02.raw Raw
BNU_A1_03.raw Raw
BNU_A2_01.raw Raw
BNU_A2_02.raw Raw
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Publications

Integrative Proteomic and Phosphoproteomic Profiling Reveals the Salt-Responsive Mechanisms in Two Rice Varieties (Oryza Sativa subsp. Japonica and Indica).

Min Cheol Woo CW   Gupta Ravi R   Lee Gi Hyun GH   Cho Jun-Hyeon JH   Kim Yu-Jin YJ   Wang Yiming Y   Jung Ki-Hong KH   Kim Sun Tae ST  

Proteomics 20241103


Salinity stress induces ionic and osmotic imbalances in rice plants that in turn negatively affect the photosynthesis rate, resulting in growth retardation and yield penalty. Efforts have, therefore, been carried out to understand the mechanism of salt tolerance, however, the complexity of biological processes at proteome levels remains a major challenge. Here, we performed a comparative proteome and phosphoproteome profiling of microsome enriched fractions of salt-tolerant (cv. IR73; indica) an  ...[more]

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