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Comparison of Myxococcus xanthus Proteomes between Wild Type and Mutant Strains

ABSTRACT: Proteomes were compared between wild type Myxococcus xanthus (DK6204) and several mutant strains to determine relative changes in protein abundance when grown under normal conditions.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Myxococcus Xanthus

SUBMITTER: Brandon Saiz

LAB HEAD: Daniel Wall

PROVIDER: PXD053716 | Pride | 2024-07-26

REPOSITORIES: Pride

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	BSA-44.raw	Raw
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Publications

Cell-cell transfer of adaptation traits benefits kin and actor in a cooperative microbe.

Subedi Kalpana K Roy Pravas C PC Saiz Brandon B Basile Franco F Wall Daniel D

Proceedings of the National Academy of Sciences of the United States of America 20240716 30

Microbes face many physical, chemical, and biological insults from their environments. In response, cells adapt, but whether they do so cooperatively is poorly understood. Here, we use a model social bacterium, <i>Myxococcus xanthus</i>, to ask whether adapted traits are transferable to naïve kin. To do so we isolated cells adapted to detergent stresses and tested for trait transfer. In some cases, strain-mixing experiments increased sibling fitness by transferring adaptation traits. This cooper ...[more]

PMID: 39012831

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LFQ Analysis of Differential Protein Regulation in Response to Tween-20 Exposure in TwnR mutant Myxococcus xanthus strains

Project description:A time series study was performed on detergent exposure in TwnR mutant (2242mut) to observe differential protein abundances between groups using label free quantitation (LFQ).

2024-07-26 | PXD053714 | Pride

LFQ Analysis of Differential Protein Regulation in Response to Tween-20 Exposure in TwnR mutant and Wild Type Myxococcus xanthus strains

Project description:This dataset compares the relative protein abundances between wild type Myxococcus xanthus and a detergent resistant mutant, in the presence or absence of 0.006% tween-20 detergent, after either 2 or 6 hours. Comparisons were made using MaxQuant and Label Free Quantitation (LFQ)

2024-07-26 | PXD053718 | Pride

Predataxis behavior in Myxococcus xanthus.

Project description:Spatial organization of cells is important for both multicellular development and tactic responses to a changing environment. We find that the social bacterium, Myxococcus xanthus utilizes a chemotaxis (Che)-like pathway to regulate multicellular rippling during predation of other microbial species. Tracking of GFP-labeled cells indicates directed movement of M. xanthus cells during the formation of rippling wave structures. Quantitative analysis of rippling indicates that ripple wavelength is adaptable and dependent on prey cell availability. Methylation of the receptor, FrzCD is required for this adaptation: a frzF methyltransferase mutant is unable to construct ripples, whereas a frzG methylesterase mutant forms numerous, tightly packed ripples. Both the frzF and frzG mutant strains are defective in directing cell movement through prey colonies. These data indicate that the transition to an organized multicellular state during predation in M. xanthus relies on the tactic behavior of individual cells, mediated by a Che-like signal transduction pathway.

| S-EPMC2579389 | biostudies-literature

Natural transformation of Myxococcus xanthus.

Project description:Myxococcus xanthus belongs to the delta class of the proteobacteria and is notable for its complex life-style with social behaviors and relatively large genome. Although previous observations have suggested the existence of horizontal gene transfer in M. xanthus, its ability to take up exogenous DNA via natural transformation has not been experimentally demonstrated. In this study, we achieved natural transformation in M. xanthus using the autonomously replicating myxobacterial plasmid pZJY41 as donor DNA. M. xanthus exopolysaccharide (EPS) was shown to be an extracellular barrier for transformation. Cells deficient in EPS production, e.g., mutant strains carrying ?difA or ?epsA, became naturally transformable. Among the inner barriers to transformation were restriction-modification systems in M. xanthus, which could be partially overcome by methylating DNA in vitro using cell extracts of M. xanthus prior to transformation. In addition, the incubation time of DNA with cells and the presence of divalent magnesium ion affected transformation frequency of M. xanthus. Furthermore, we also observed a potential involvement of the type IV pilus system in the DNA uptake machinery of M. xanthus. The natural transformation was totally eliminated in the ?pilQ/epsA and ?tgl/epsA mutants, and null mutation of pilB or pilC in an ?epsA background diminished the transformation rate. Our study, to the best of our knowledge, provides the first example of a naturally transformable species among deltaproteobacteria.

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Accordion waves in Myxococcus xanthus.

Project description:Myxococcus xanthus are Gram-negative bacteria that glide on solid surfaces, periodically reversing their direction of movement. When starved, M. xanthus cells organize their movements into waves of cell density that sweep over the colony surface. These waves are unique: Although they appear to interpenetrate, they actually reflect off one another when they collide, so that each wave crest oscillates back and forth with no net displacement. Because the waves reflect the coordinated back and forth oscillations of the individual bacteria, we call them "accordion" waves. The spatial oscillations of individuals are a manifestation of an internal biochemical oscillator, probably involving the Frz chemosensory system. These internal "clocks," each of which is quite variable, are synchronized by collisions between individual cells using a contact-mediated signal-transduction system. The result of collision signaling is that the collective spatial behavior is much less variable than the individual oscillators. In this work, we present experimental observations in which individual cells marked with GFP can be followed in groups of unlabeled cells in monolayer cultures. These data, together with an agent-based computational model demonstrate that the only properties required to explain the ripple patterns are an asymmetric biochemical limit cycle that controls direction reversals and asymmetric contact-induced signaling between cells: Head-to-head signaling is stronger than head-to-tail signaling. Together, the experimental and computational data provide new insights into how populations of interacting oscillators can synchronize and organize spatially to produce morphogenetic patterns that may have parallels in higher organisms.

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