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Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses

ABSTRACT: Mitosis in early embryos often proceeds at a rapid pace, but how this pace is achieved is not understood. Here we show that cyclin B3 is the dominant driver of rapid embryonic mitoses in the C. elegans embryo. Cyclins B1 and B2 support slow mitosis (NEBD to Anaphase ~600s) but the presence of cyclin B3 dominantly drives the ~3-fold faster mitosis observed in wildtype. Multiple mitotic events are slowed down in Cyclin B1&B2-driven mitosis and cyclin B3-associated Cdk1 H1 kinase activity is ~25-fold more active than cyclin B1-associated Cdk1. Addition of cyclin B1 to fast cyclin B3-only mitosis introduces an ~60s delay between completion of chromosome alignment and anaphase onset; this delay, which is important for segregation fidelity, is dependent on inhibitory phosphorylation of the anaphase activator Cdc20. Thus, cyclin B3 dominance, coupled to a cyclin B1-dependent delay that acts via Cdc20 phosphorylation, sets the rapid pace and ensures mitotic fidelity in the early C. elegans embryo.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Embryo, Whole Body

SUBMITTER: Pablo Lara-Gonzalez

LAB HEAD: Pablo Lara-Gonzalez

PROVIDER: PXD053811 | Pride | 2024-07-29

REPOSITORIES: Pride

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			Action	DRS
	PLG_01.raw	Raw
	PLG_01_protein-peptides.csv	Csv
	PLG_02.raw	Raw
	PLG_02_protein-peptides.csv	Csv
	PLG_03.raw	Raw

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