Proteomics

Dataset Information

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HDX-MS analysis of PTPN2 with the CRBN/DDB1 complex

ABSTRACT: We used HDX-MS analysis to analyze the interaction between PTPN2 with a compound both in the presence and absence of the CRBN/DDB1 complex. We identified significant changes in HDX when PTPN2 was in the presence of compound. We also identified significant changes when comparing the deuteration profiles of PTPN2 in the presence of compound with and without the CRBN/DDB1 complex.

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: Dr. John E. Bruke

PROVIDER: PXD053867 | Pride | 2024-08-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
11_09_23_Calico6_PT_MSMS.d.rar Other
12_07_23_HNHDX10_DB_3s-1.d.rar Other
12_07_23_HNHDX10_PC_30s-1.d.rar Other
12_07_23_HNHDX10_PC_3s-1.d.rar Other
12_07_23_HNHDX10_TN_300s-1.d.rar Other
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Publications

PTPN2 (protein tyrosine phosphatase non-receptor type 2, or TC-PTP) and PTPN1 are attractive immuno-oncology targets, with the deletion of Ptpn1 and Ptpn2 improving response to immunotherapy in disease models. Targeted protein degradation has emerged as a promising approach to drug challenging targets including phosphatases. We developed potent PTPN2/N1 dual heterobifunctional degraders (Cmpd-1 and Cmpd-2) which facilitate efficient complex assembly with E3 ubiquitin ligase CRL4<sup>CRBN</sup>,  ...[more]

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