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A platform for mapping reactive cysteines within the immunopeptidome

ABSTRACT: The major histocompatibility complex class I (MHC-I) antigen presentation pathways play pivotal roles in orchestrating immune responses. Recent studies have begun to utilize cysteines within the immunopeptidome for therapeutic applications, such as using covalent ligands to create haptenated neoantigens for inducing an immune response. In this study, we report a platform for mapping reactive cysteines present on MHC-I-bound peptide antigens. We have developed cell-impermeable sulfonated maleimide probes capable of effectively capturing reactive cysteines on antigens. Utilizing these probes in chemoproteomic experiments, we discovered that cysteines on MHC-I-bound antigens exhibit various degrees of reactivity. Furthermore, interferon-gamma stimulation produces increased reactivity of cysteines at position 8 of 9-mer MHC-I-bound antigens. Our findings may open up new avenues for understanding the distinctive roles of cysteine within the MHC-I immunopeptidome and leveraging the differentially reactive cysteines for therapeutic intervention.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, T Cell, Cell Culture

DISEASE(S): B-lymphoblastic Leukemia/lymphoma

SUBMITTER: Xiaoyu Zhang

LAB HEAD: Xiaoyu Zhang

PROVIDER: PXD054678 | Pride | 2024-10-17

REPOSITORIES: Pride

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			Action	DRS
	BV173WTvsHLAKO_6plex_01.raw	Raw
	BV173WTvsHLAKO_6plex_02.raw	Raw
	BV173WTvsHLAKO_6plex_03.raw	Raw
	BV173WTvsHLAKO_6plex_04.raw	Raw
	BV173WTvsHLAKO_6plex_05.raw	Raw

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