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Integrative phosphoproteomic analysis reveal hemstatic-endothelial singaling internplay

ABSTRACT: The vascular endothelial cell (EC) monolayer plays a crucial part in maintaining hemostasis. An extensive array of G protein-coupled receptors (GPCRs) allows ECs to dynamically act on key hemostatic stimuli such as thrombin and histamine. The impact of these individual stimuli on EC signal transduction has been the subject of various studies, but insight into discordant and concordant EC signaling between different GPCRs remain limited. Using mass spectrometry- based phosphoproteomics, we delineated the unbiased signaling response for histamine stimulation in the endothelium and observed highly similar responses between different protease activated receptors (PAR1-4). Finally, we compare both histamine and PAR agonists, indicating uniquely activated proteins between both stimuli, but a general overlapping activation of cell-junction and actin cytoskeletal proteins. Thus, we provide an integrative phosphoproteomic analysis of histamine and PAR agonist in the endothelium that highlights the endothelial response programs that are at the bases of regulating hemostasis.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Colony Forming Cell, Endothelial Cell

DISEASE(S): Von Willebrand's Disease 2,Von Willebrand's Disease 1

SUBMITTER: Stijn Groten

LAB HEAD: Arjan Hoogendijk

PROVIDER: PXD055122 | Pride | 2024-11-27

REPOSITORIES: Pride

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			Action	DRS
	240730_SG_HisThr_Prot_THR_10_R2_S1-A10_1_10656.d.zip	Other
	DIANN_analysis.zip	Other
	checksum.txt	Txt

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Integrative phosphoproteomic analysis reveal hemstatic-endothelial singaling internplay

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