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Global proteomics indicates subcellular-specific anti-ferroptotic responses to ionizing radiation

ABSTRACT: Cells have many protective mechanisms against background levels of ionising radiation (IR) orchestrated by molecular changes in expression, post-translation modifications and subcellular localisation. Radiotherapeutic treatment in oncology attempts to overwhelm such mechanisms, but radio-resistance is an ongoing challenge. Here, global subcellular proteomics combined with Bayesian modelling identified 544 differentially localised proteins in A549 cells upon 6 Gy x-ray exposure, revealing subcellular-specific changes of proteins involved in ferroptosis, an iron-dependent cell death, suggestive of potential radio-resistance mechanisms. These observations were independent of expression changes, emphasizing the utility of global subcellular proteomics and the promising prospect of ferroptosis-inducing therapies for combatting radioresistance.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Josie Christopher

LAB HEAD: Kathryn S. Lilley

PROVIDER: PXD055123 | Pride | 2024-12-16

REPOSITORIES: Pride

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			Action	DRS
	Phospho_TiO2_F.msf	Msf
	Phospho_TiO2_F.msfView	Msf
	Phospho_TiO2_F.pdResult	Other
	Phospho_TiO2_F.pdResultView	Other
	Phospho_TiO2_F.pdStudy	Other

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