Validation of potential growth-related proteins in chick vitreous during emmetropization using targeted-based proteomics (MRMHR)
Ontology highlight
ABSTRACT: The relatively new high-resolution MRM (MRMHR) approach with the Triple-TOF MS running under nanoflow (Which is similar to the SRM/MRM approach) was adopted in this study to solidify further the findings of SWATH-MS results of differentially expressed proteins found across the time points in the chick vitreous for the benefit of low peptide amount consumption. Twelve up-regulated and 26 down-regulated proteins were found across all time points compared to day 7 using SWATH-MS. Several DEPs, such as alpha-fetoprotein, cadherins family group, neurocan, and reelin involved in structural and growth-related pathways, were validated for the first time using MRMHR under this experimental condition.
INSTRUMENT(S): TripleTOF 6600
ORGANISM(S): Gallus Gallus (chicken)
TISSUE(S): Whole Body
SUBMITTER: Jimmy Ka-wai Cheung
LAB HEAD: Jimmy Ka-Wai
PROVIDER: PXD056097 | Pride | 2024-09-24
REPOSITORIES: Pride
ACCESS DATA