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Development of a proximity labeling vector to identify protein-protein interactions in human stem cells

ABSTRACT: Development a novel system employing TurboID-fusion proteins within an integrative and inducible expression vector to investigate the interactome during stem cell differentiation. Cells expressing U2AF2 (iU2) or GFP (iGFP) fused with TurboID in undifferentiated (D0) or mesoderm differentiated (D1) were analyzed to identified the interactome and detect changes under differentiation process.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell, Embryonic Stem Cell

SUBMITTER: Rubens Gomes Junior

LAB HEAD: Bruno Dallagiovanna

PROVIDER: PXD056299 | Pride | 2025-01-07

REPOSITORIES: pride

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			Action	DRS
	20231228_243823_rubens_iU2D0R1_1.raw	Raw
	20231228_243823_rubens_iU2D0R1_2.raw	Raw
	20231228_243923_rubens_iU2D0R2_1.raw	Raw
	20231228_243923_rubens_iU2D0R2_2.raw	Raw
	20231228_244023_rubens_iU2D0R3_1.raw	Raw

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