Proteomics

Dataset Information

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Human CD8 T cell IL2 deprivation time course


ABSTRACT: IL2 deprivation time course for day 8 human CD8 T cells activated for 3 days with TransAct T-cell activation beads. PBMC (Cambridge Bio) were defrosted and CD8+ T-cells isolated using a human CD8+ T-cell isolation kit. CD8+ T-cells were activated at 1 x 106 cells/mL with 1 in 100 TransAct T-cell activation beads (Miltenyi) and 20 ng/mL IL-2 in complete RMPI (RMPI 1640, 10% FBS, penicillin/streptomycin) for three days. Cells were then washed out of activation and then maintained in complete RMPI with 20 ng/mL IL-2 for five days. To remove IL-2 for IL-2 deprivation, cells were washed twice in pre-warmed media, then replated in complete media without IL-2. Cells were deprived of IL-2 for 1hr, 3hrs, 5hrs, 8hrs, 24hrs or 48hrs.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peripheral Blood Lymphocyte

SUBMITTER: Alejandro Brenes  

LAB HEAD: Doreen A Cantrell

PROVIDER: PXD056475 | Pride | 2024-10-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
238_2023_DUN_DAC-SP-1A-Rep.raw Raw
238_2023_DUN_DAC-SP-1A.raw Raw
238_2023_DUN_DAC-SP-1B-1HRS-Rep.raw Raw
238_2023_DUN_DAC-SP-1B-1HRS.raw Raw
238_2023_DUN_DAC-SP-1C-3HRS-Rep.raw Raw
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