Quantitative proteomics reveals inflammatory signaling in golgin-97 knockout-mediated breast cancer progression

ABSTRACT: The Golgi apparatus serves as a central hub for trafficking, regulating the anterograde transport and modification of proteins and lipids, or recycling extracellular proteins via retrograde transport through endosomes. Several Golgi-localized proteins have been reported to be involved in regulating cancer cell migration and invasion. Our previous study showed that the Golgi tethering factor golgin-97 is downregulated in breast cancer and is correlated with patient prognosis. Here, we demonstrated that the knockout of golgin-97 promotes tumor malignancy in breast cancer in vivo. We identified differentially expressed proteins between golgin-97 knockout cells and their parental breast cells using a quantitative proteomic approach based on 6-plex tandem mass tags (TMT) technology. Bioinformatics analysis further revealed that golgin-97 knockout was significantly related to inflammation-related factors and MAPK signaling pathways. We also integrated proteomic and genomic datasets to explore the biological impacts of golgin-97 in regulating breast cancer progression.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Chia-Jung Yu  

LAB HEAD: Chia-Jung Yu

PROVIDER: PXD056499 | Pride | 2025-03-07

REPOSITORIES: Pride