Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

(Phospho-)proteomics analysis of fission yeast cells during mating and cell-cell fusion

ABSTRACT: In fission yeast Schizosaccharomyces pombe, sexual reproduction is triggered by the absence of nitrogen (N), and relies on crosstalk between multiple signaling pathways, with the inactivation of the TORC1 complex playing a critical role. This process is further elicited by pheromone signaling between the two mating types, P (h+) and M (h-), through GPCR activation of a Ras-MAPK cascade. Once partners are selected, cell projections (shmoos) extend to bring the cells together. Upon pairing, actin fusion foci at the contact site, concentrate hydrolases to digest the cell wall and ensure coordinated fusion. While several kinases and phosphatases are known to promote sexual differentiation and cell fusion, their specific substrates remain largely unknown. To address those questions, we have developed methods to synchronize cell populations during mating and cell-cell fusion and used time course phospho-proteomics to identify dynamic phosphorylation changes. The aim was to uncover the phosphorylation events driving differentiation and morphogenetic changes necessary for cell projection and cell fusion.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Schizosaccharomyces Pombe 972h-

SUBMITTER: Sophie Martin

LAB HEAD: Martin Sophie

PROVIDER: PXD056619 | Pride | 2024-12-06

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	210211_Berard_TMT_Phospho_12944.raw	Raw
	210211_Berard_TMT_Phospho_12944_CE40.raw	Raw
	210216_Berard_TMT_12965_phospho.raw	Raw
	210216_Berard_TMT_12965_phospho_CE40.raw	Raw
	210222_Berard_12965_TMT_CV40-60.raw	Raw

Items per page:

1 - 5 of 40

Similar Datasets

A velvet transcription factor specifically activates mating through a novel surface protein in the human fungal pathogen Cryptococcus deneoformans

Project description:Sexual reproduction facilitates infection by the production of both a lineage advantage and infectious sexual spores in the ubiquitous human fungal pathogen Cryptococcus deneoformans. However, the regulatory determinants specific for initiating mating remain poorly understood. Here, we identified a velvet family regulator, Cva1, that strongly promotes sexual reproduction in C. deneoformans. This regulation was determined to be specific, based on a comprehensive phenotypic analysis of cva1 under 25 distinct in vitro and in vivo growth conditions. We further revealed that Cva1 plays a critical role in the initiation of early mating events, especially sexual cell-cell fusion, but is not important for the late sexual development stages or meiosis. Thus, Cva1 specifically contributes to mating activation. Importantly, a novel mating-responsive surface protein, Cfs1, serves as the key target of Cva1 during mating, since its absence nearly blocks cell-cell fusion in C. deneoformans and its sister species C. neoformans. Together, our findings provide insight into how C. deneoformans ensures regulatory specificity of mating.

2022-02-10 | GSE196267 | GEO

Glucose depletion enables Candida albicans mating independent of the epigenetic white-opaque switch

Project description:The human fungal pathogen Candida albicans can switch stochastically and heritably between a “white” phase and an “opaque” phase. Opaque cells are the mating-competent form of the species whereas white cells are essentially “sterile”. Here, we report that glucose depletion, a common nutrient stress, enables C. albicans white cells to undergo efficient sexual mating. The relative expression levels of pheromone-sensing and mating-associated genes (including STE2/3, MFA1, MFalpha1, FIG1, FUS1, and CEK1/2) were increased under glucose depletion conditions, while expression of mating repressors TEC1 and DIG1 was decreased. We show that Cph1 and Tec1, factors that act downstream of the pheromone MAPK pathway, play opposite roles in regulating white cell mating as TEC1 deletion or CPH1 overexpression promoted white cell mating. Moreover, inactivation of the Cph1 repressor Dig1 increased white cell mating ~4,000 fold in glucose-depleted medium relative to that in the presence of glucose. These findings reveal that the white-to-opaque epigenetic switch may not be a prerequisite for sexual mating in C. albicans in nature. Given parallels between C. albicans white cell mating to that of other yeast species, this mechanism of mating could represent a more ancient strategy of sexual reproduction in C. albicans.

2023-03-03 | PXD040559 |

White Cells Facilitate Opposite- and Same-Sex Mating of Opaque Cells in Candida albicans

Project description:Modes of sexual reproduction in eukaryotic organisms are highly diversified. The human fungal pathogen Candida albicans undergoes a phenotypic switch from the white to the opaque phase in order to become mating-competent. In this study, we report that functionally and morphologically differentiated white and opaque cells show a coordinating behavior in the process of mating. Although white cells are mating-incompetent, they are induced to produce sexual pheromones when treated with opposite pheromones or interacted with opaque cells of an opposite mating type. In a co-culture system, pheromones released by white cells induce opaque cells to form mating projections and thus facilitate both opposite- and same-sex mating of opaque cells. Deletion of genes encoding the pheromone precursor proteins and inactivation of the pheromone response signaling pathway (Ste2-MAPK-Cph1) impair the promoting role of white cells (MTLa) in sexual mating of opaque cells. White and opaque cells communicate via a paracrine pheromone signaling and thus create an environment conducive to sexual mating. This coordination behavior of the two different cell types may be a trade-off strategy between sexual and asexual lifestyles in C. albicans.

2014-10-23 | GSE56039 | GEO

White Cells Facilitate Opposite- and Same-Sex Mating of Opaque Cells in Candida albicans

2014-10-23 | E-GEOD-56039 | biostudies-arrayexpress

Transcription profiling of honey bee queens treated with carbon dioxide and physical manipulation vs virgin controls

Project description:Mating is a complex process that causes many behavioral and physiological changes, but the factors triggering these changes and the underlying molecular processes are not well characterized. Honey bee queens provide a convenient system for dissecting these factors (e.g., physical manipulation, insemination volume, insemination substance) via instrumental insemination. We examined the effects of carbon dioxide (CO2), a commonly used anesthetic in instrumental insemination that causes changes similar to those observed after mating, and physical manipulation, which presumably mimics the act of copulation, on the brain transcriptional changes in honey bee queens. We found significant gene overlap between our study and previous mating studies in honey bee queens and Drosophila. This suggests that molecular pathways regulating the mating process are conserved across different mating regimes of honey bees as well as across insect orders.

2011-01-30 | E-MEXP-2646 | biostudies-arrayexpress

External and intrinsic mechanical stress restricts pheromone signaling by direct phosphorylation of the Ste5 scaffold by Pkc1

Project description:Ste5 and Far1 function as prototypical scaffold proteins activating a MAP-kinase cascade and polarizing yeast cells in response to pheromones. Here we show that Pkc1-dependent phosphorylation of conserved serine residues in their RING-H2 domains down-regulates pheromone induced signaling upon mechanical stress. Phosphorylation of Ste5 on serine 185 by Pkc1 interferes with its membrane-association in vivo by preventing binding to the receptor linked Gbg protein as determined by in vitro assays. Quantitative readouts of pheromone signaling show that Pkc1 induces rapid dispersal of Ste5 from mating projections upon mechanically-induced cell wall stress as well as during cell-cell fusion, leading to inhibition of MAPK activity and polarized growth. Cells expressing non-phosphorylatable Ste5 (Ste5S185A) undergo increased lysis during mechanical stress and cell-cell fusion compared to wild-type controls, and this effect is exacerbated by simultaneous expression of non-phosphorylatable Far1 (Far13A). Taken together, these results uncover a cross-talk mechanism explaining how mating cells inhibit pheromone signaling upon extrinsic or intrinsic mechanical stress to orchestrate polarized growth and cell-cell fusion.

2019-06-17 | PXD004657 | Pride

Discovery of a phenotypic switch regulating sexual mating in the opportunistic fungal pathogen Candida tropicalis

Project description:Sexual reproduction can promote genetic diversity in eukaryotes, and yet many pathogenic fungi have been labeled as obligate asexual species. It is becoming increasingly clear, however, that cryptic sexual programs may exist in some species, and that efficient mating requires the necessary developmental switch to be triggered. In this study we investigate Candida tropicalis, an important human fungal pathogen that has been reported to be asexual. Significantly, we demonstrate that C. tropicalis uses a phenotypic switch to regulate a cryptic program of sexual mating. Thus, diploid a and α cells must undergo a developmental transition to the mating-competent form, and only then does efficient cell-cell conjugation take place resulting in the formation of stable a/α tetraploids. We show that both the phenotypic switch and sexual mating depend on the conserved transcriptional regulator Wor1, which is regulated by temperature in other fungal species. In contrast, C. tropicalis mating occurs efficiently at both 25 °C and 37 °C, suggesting that it could occur in the mammalian host and have direct consequences for the outcome of an infection. Transcriptional profiling further reveals that ≈400 genes are differentially expressed between the two phenotypic states, including the regulatory factor Wor1. Taken together, our results demonstrate that C. tropicalis has a unique sexual program, and that entry to this program is controlled via a Wor1-mediated, metastable switch. These observations have direct implications for the regulation and evolution of cryptic sexual programs in related fungal pathogens.

2012-08-28 | GSE40179 | GEO

pH regulates white-opaque switching and sexual mating in Candida albicans

Project description:As a successful commensal and pathogen of humans, Candida albicans encounters a wide range of environmental changes. Among them, ambient pH is an important factor, which changes frequently and affects many biological processes in this species. The ability to adapt to pH changes is tightly linked with pathogenesis and morphogenesis. In this study, we report that pH has a profound effect on white-opaque switching and sexual mating in C. albicans. Acidic pHs promote white-to-opaque switching but repress sexual mating of opaque cells. The cAMP signaling and Rim101-mediated pH sensing pathways are involved in the regulation of pH-regulated white-opaque switching. Interestingly, white and opaque cells of the cyr1/cyr1 mutant, which is defective in producing cAMP, show distinct growth defects under acidic and alkaline conditions. Phr2 could play a major role in acidic pHs-induced opaque cell formation. We further discover that acidic pH conditions repress sexual mating due to the failure of activation of the Ste2-mediated a-pheromone response pathway. The effects of pH changes on phenotypic switching and sexual mating could be a balance behavior between host adaptation and sexual reproduction.

2015-12-30 | GSE67101 | GEO

Project description:SsUBC2 gene regulates sexual mating

| PRJNA855015 | ENA

Global transcriptome analysis of heterodimeric homeobox-driven zygote developmental program in Chlamydomonas reinhardtii

Project description:Chlamydomonas reinhardtii provides an important model to study sexual reproduction in a unicellular context. Its sexual cycle culminates in the formation of diploid zygotes which differentiate into dormant spores that eventually undergo meiosis. Mating between haploid plus and minus gametes induces rapid cell wall shedding via the enzyme g-Lysin; cell fusion is followed by by heterodimerization of sex-specific homeobox transcription factors, Gsm1 and Gsp1, and initiation of zygote-specific gene expression. Developing zygotes secrete novel cell-wall materials that create multicellular aggregates called pellicle. To investigate the genetic underpinnings of zygote differentiation, we performed comparative transcriptome analysis of both pre- and post-mating samples. We identified 253 genes specifically enriched in early zygotes, 92% of which are not expressed in gsp1 null zygotes, indicating that the Gsm1/Gsp1 heterodimer is the main activator of the early zygote program. A cohort of genes induced by g-Lysin is shown to be negatively regulated in early zygotes at the post-transcriptional level in a Gsm1/Gsp1-dependent manner, enabling prompt transition to zygotic wall assembly.

2017-08-29 | GSE91400 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data