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Dictyostelium discoideum O-Thr-fucome from WT and SPY-KO developing cells (slugs)

ABSTRACT: Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO developing cells (slugs) were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

TISSUE(S): Cell Culture

SUBMITTER: Christopher West

LAB HEAD: Christopher M. West

PROVIDER: PXD056866 | Pride | 2025-01-30

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	AX3_1B_FOS_T_3_5ul_5Mar24_1.raw	Raw
	AX3_1B_FOS_T_3_5ul_5Mar24_2.raw	Raw
	AX3_1B_FOS_T_3_5ul_5Mar24_3.raw	Raw
	AX3_FOT_slug_4_75ul_26Apr24_1.raw	Raw
	AX3_FOT_slug_4_75ul_26Apr24_2.raw	Raw

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Project description:Dictyostelium discoideum O-Ser and O-Thr fucosylated proteins from WT, SPY-OE and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser and anti O-Thr fucose polyclonal antibodies in 3 Technical Replicates, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation), and SPY_OE (mutant, SPY overexpressor strain).

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