Dataset Information

Proteomics analysis of lysine methylated proteins from Leishmania donovani strain 1S: wild type and set mutants

ABSTRACT: This study was performed with the goal of understanding the roles of Leishmania donovani SET7 and SET29 proteins. Using homologous recombination, set7 and set29 genes were knocked out, thereby creating set7 and set29 mutant parasites. Whole cell extracts were isolated from wild type parasites, set7 mutant parasites and set29 mutant parasites, followed by immunoprecipitation of lysine methylated proteins using anti-methyllysine antibody. The immunoprecipitated proteins were identified by LC-MS analysis

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Leishmania Donovani

SUBMITTER: Swati Saha

LAB HEAD: Swati Saha

PROVIDER: PXD057513 | Pride | 2025-01-24

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	Experiment2_set29mutant.msf	Msf
	Experiment2_set29mutant.raw	Raw
	Experiment2_set7mutant.msf	Msf
	Experiment2_set7mutant.raw	Raw
	Experiment2_wildtype.msf	Msf

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Publications

The SET29 and SET7 proteins of Leishmania donovani exercise non-redundant convergent as well as collaborative functions in moderating the parasite's response to oxidative stress.

Sharma Varshni V Pal Jyoti J Dashora Vishal V Chattopadhyay Somdeb S Kapoor Yogita Y Singha Biplab B Arimbasseri G Aneeshkumar GA Saha Swati S

The Journal of biological chemistry 20250120

SET proteins are lysine methyltransferases. In investigating Leishmania donovani SET29, we found depletion of LdSET29 by two-thirds did not affect promastigote growth, nor alter the parasite's response to UV-induced or HU-induced stress, but made it more tolerant to H<sub>2</sub>O<sub>2</sub>-induced oxidizing environment. The deviant response to oxidative stress was coupled to lowered accumulation of reactive oxygen species, which was linked to enhanced scavenging activity. The set29 mutants' r ...[more]

PMID: 39842664

Similar Datasets

Project description:This study was performed with the goal of understanding the roles of Leishmania donovani SET7 and SET29 proteins in gene regulation. Using homologous recombination to knock out set7 and set29 genes, set7 and set29 mutant parasites were created. This was folllowed by transcriptome analyses of wild type parasites, set7 mutant parasites and set29 mutant parasites, that were carried out using RNA-seq. In the first experimental set, transcriptomes of wild type parasites cultured under normal conditions as well as under hydrogen peroxide (H2O2) -induced oxidizing conditions were analysed, along with transcriptomes of set7 mutant parasites that were similarly grown under normal growth conditions as well as under H2O2-induced oxidizing conditions. Using the data obtained, the transcriptome of wild type parasites exposed to H2O2 has been compared with the transcriptome of wild type parasites grown under normal conditions. The transcriptome of set7 mutant parasites grown under H2O2-induced oxidizing conditions versus normal conditions have been similarly compared. In addition, the transcriptome of set7 mutant parasites grown under normal conditions has been compared to that of similarly grown wild type parasites. In the second experimental set, transcriptomes of wild type parasites cultured under normal growth conditions, set29 mutant parasites grown under normal conditions, as well as set29 mutant parasites grown under H2O2-induced oxidizing conditions, were analysed. Using the data obtained, the transcriptome of set29 mutant parasites grown under normal conditions has been compared to that of similarly grown wild type parasites. The transcriptome of set29 mutant parasites grown under H2O2-induced oxidizing conditions versus normal conditions have been similarly compared. Our data reveals, that knock out of set7 modulates transcription of specific clusters of genes on particular chromosomes, with almost 10 % of the total genes being regulated. On the other hand, depletion of SET29 modulates transcription of a very small set of genes distributed primarily in two clusters. This datasheet is for second experimental set.