Proteomics

Dataset Information

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Mycobacterium abscessus cultured in SCFM with heme treatment.


ABSTRACT: Mycobacterium abscessus (Mabs) biofilms are altered in the presence of exogenous heme. We wanted to determine how protein levels were affected by heme. Mabs cells were grown in a modified media formulated to mimic the nutritional environment of the sputum of cystic fibrosis (CF) patients. Mabs cells grown in synthetic CF media (SCFM) typically form submerged biofilms, exogenous heme treatment however prevents this film formation and supports growth of a pellicle at the air-liquid interface. Heme treatment reduced several iron uptake and homeostasis proteins, suggesting heme is acting as an iron source for these bacteria. In addition, multiple proteins were upregulated in heme treatment, however, the function of these proteins remains to be determined.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacteria Mycobacterium Abscessus (strain Atcc 19977 / Dsm 44196 / Cip 104536 / Jcm 13569 / Nctc 13031 / Tmc 1543)

SUBMITTER: Rajesh Soni  

LAB HEAD: Rebecca Donegan

PROVIDER: PXD057673 | Pride | 2025-02-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
RD1313_Fusion_DDA_Maxquant_ProteinGroups.xlsx Xlsx
RD1313_Fusion_DIA_H1.raw Raw
RD1313_Fusion_DIA_H2.raw Raw
RD1313_Fusion_DIA_H3.raw Raw
RD1313_Fusion_DIA_HB1.raw Raw
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