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Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota

ABSTRACT: Marine brown algae produce the highly recalcitrant polysaccharide fucoidan, contributing to long-term oceanic carbon storage and climate regulation. Fucoidan is degraded by specialized heterotrophic bacteria, which promote ecosystem function and global carbon turnover using largely uncharacterized mechanisms. Here, we isolate and study two Planctomycetota strains from the microbiome associated with the alga Fucus spiralis, which grow efficiently on chemically diverse fucoidans. One of the strains appears to internalize the polymer, while the other strain degrades it extracellularly. Multi-omic approaches show that fucoidan breakdown is mediated by the expression of divergent polysaccharide utilization loci, and endo-fucanases of family GH168 are strongly upregulated during fucoidan digestion. Enzymatic assays and structural biology studies reveal how GH168 endo-fucanases degrade various fucoidan cores from brown algae, assisted by auxiliary hydrolytic enzymes. Overall, our results provide insights into fucoidan processing mechanisms in macroalgal-associated bacteria.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Planctomycetales

SUBMITTER: Mikel Azkargorta

LAB HEAD: Felix Elortza

PROVIDER: PXD057797 | Pride | 2024-11-22

REPOSITORIES: Pride

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			Action	DRS
	230721_230622B_CarlaAzti_Pellets_10_S2-A10_1_9849.RAW	Raw
	230721_230622B_CarlaAzti_Pellets_11_S2-A11_1_9850.RAW	Raw
	230721_230622B_CarlaAzti_Pellets_12_S2-A12_1_9851.RAW	Raw
	230721_230622B_CarlaAzti_Pellets_13_S2-B1_1_9852.RAW	Raw
	230721_230622B_CarlaAzti_Pellets_14_S2-B2_1_9853.RAW	Raw

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