Proteomics

Dataset Information

0

SILAC of hESCs with DDX18 knockdown


ABSTRACT: This study explores the crucial role of the nucleolus-specific RNA helicase DDX18 in maintaining the pluripotency of human embryonic stem cells. We demonstrate that DDX18 coordinates nucleolus phase separation and chromatin organization by interacting with NPM1 in the granular component of the nucleolus, facilitated by nucleolar RNA species. The absence of DDX18 disrupts immiscible nucleolar substructures, significantly affecting centromere clustering and perinucleolar heterochromatin (PNH) formation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Xin Huang  

LAB HEAD: Jianlong Wang

PROVIDER: PXD057813 | Pride | 2024-11-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MS191587-L1-.msf Msf
MS191587-L1-1.raw Raw
MS191587-L1-10.raw Raw
MS191587-L1-11.raw Raw
MS191587-L1-12.raw Raw
Items per page:
1 - 5 of 27

Similar Datasets

2014-03-26 | E-GEOD-56186 | biostudies-arrayexpress
2011-04-22 | E-GEOD-28813 | biostudies-arrayexpress
2015-03-06 | E-GEOD-59792 | biostudies-arrayexpress
2024-10-17 | PXD051121 | Pride
2024-10-01 | GSE232870 | GEO
2024-10-01 | GSE232871 | GEO
2015-09-14 | PXD001391 | Pride
2024-04-17 | GSE249545 | GEO
2017-06-12 | PXD006093 | Pride
2017-06-18 | GSE78043 | GEO