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In-Cell Fast Photochemical Oxidation of Integral Membrane Proteins

ABSTRACT: We introduce IC-FPOMP (in-cell fast photochemical oxidation of MPs), a method enabling in situ footprinting of IMPs within live cells. IC-FPOMP generates reactive oxygen radicals from several precursors nearby the membrane. We achieve high-throughput and rapid footprinting of IMPs. IC-FPOMP of two human IMPs (human glucose transporter-hGLUT1 and human gamma glutamyl carboxylase-hGGCX) were successful, providing comprehensive footprinting of both the transmembrane and extramembrane regions. IC-FPOMP offers insights on IMP structure-function relationships in cell for the first time, facilitating drug discovery.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Disease Free

SUBMITTER: Jie Sun

LAB HEAD: Michael L.

PROVIDER: PXD058347 | Pride | 2025-02-14

REPOSITORIES: pride

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			Action	DRS
	4_HGGCX_CELL_33uL_H2O2_3.raw	Raw
	5_HGGCX_CELL_33uL_TiO2_3.raw	Raw
	HGGCX_CEL_4_33uL_20230407_H2O2_2.raw	Raw
	HGGCX_CEL_4_33uL_H2O2_1.raw	Raw
	HGGCX_CEL_5_33uL_TiO2_1.raw	Raw

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