Proteomics

Dataset Information

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One-carbon fixation via the reductive glycine pathway exceeds yield of the Calvin cycle in bacteria


ABSTRACT: One-carbon (C1) feedstocks like formate could be energetically efficient substrates for sustainable microbial production of food, fuels and chemicals. Here, we replace the native energy-inefficient Calvin-Benson-Bassham (CBB) cycle in Cupriavidus necator with the more energy-efficient reductive glycine pathway for growth on formate and CO2. In chemostats, our engineered strain reaches a 17% higher biomass yield than the wild type, or any natural formatotroph using the Calvin cycle. This demonstrates the potential of synthetic metabolism to realize sustainable, bio-based production.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Cupriavidus Necator (strain Atcc 17699 / H16 / Dsm 428 / Stanier 337) (ralstonia Eutropha)

TISSUE(S): Cell Culture

SUBMITTER: Timo Glatter  

LAB HEAD: Timo Glatter

PROVIDER: PXD059545 | Pride | 2025-02-15

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
THF-2-01re2.raw Raw
THF-2-02re2.raw Raw
THF-2-03re.raw Raw
THF-2-04re.raw Raw
THF-2-05re.raw Raw
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