One-carbon fixation via the reductive glycine pathway exceeds yield of the Calvin cycle in bacteria
Ontology highlight
ABSTRACT: One-carbon (C1) feedstocks like formate could be energetically efficient substrates for sustainable microbial production of food, fuels and chemicals. Here, we replace the native energy-inefficient Calvin-Benson-Bassham (CBB) cycle in Cupriavidus necator with the more energy-efficient reductive glycine pathway for growth on formate and CO2. In chemostats, our engineered strain reaches a 17% higher biomass yield than the wild type, or any natural formatotroph using the Calvin cycle. This demonstrates the potential of synthetic metabolism to realize sustainable, bio-based production.
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Cupriavidus Necator (strain Atcc 17699 / H16 / Dsm 428 / Stanier 337) (ralstonia Eutropha)
TISSUE(S): Cell Culture
SUBMITTER:
Timo Glatter
LAB HEAD: Timo Glatter
PROVIDER: PXD059545 | Pride | 2025-02-15
REPOSITORIES: pride
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