Proteomics

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Mouse islet ATG7 beta-cell knockout LC-MS/MS


ABSTRACT: We knocked out the critical autophagy enzyme, ATG7, in the β-cells of mice (ATG7Δβ-cell) then monitored blood glucose to assess the phenotype induced by this KO model. We found that all ATG7Δβ-cell mice developed diabetes between 11-15 weeks of age. We isolated islets from ATG7Δβ-cell and littermate control mice several weeks prior to diabetes development (7-10 weeks of age) and performed bulk islet proteomics. The purpose of this experiment was to understand the islet biological process pathways altered by dysfunctional β-cell autophagy in the ATG7Δβ-cell model.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Pancreatic Islet, Islet Of Langerhans

SUBMITTER: Matt Austin  

LAB HEAD: Amelia K. Linnemann

PROVIDER: PXD060032 | Pride | 2025-01-22

REPOSITORIES: pride

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