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Identification of global proteome changes upon rapalog-induced tethering of WIPI2 to the mitochondrial outer membrane in wild-type or NIX/BNIP3 DKO HeLa cells

ABSTRACT: We aimed to identify proteins that would be turned over upon artificial tethering of WIPI2 to the mitochondrial surface upon rapalog treatment, using the FIS1-FRB plus FKBP-GFP-WIPI2 tethering system. We had previously observed that this induces mitophagy based on flow cytometry analysis of the mt-mKeima probe. Here, we aimed to identify which proteins are degraded upon 24 h Rapalog treatment and verified whether there was an enrichment of mitochondrial proteins being selectively turned over by mitophagy under these conditions in wild-type and NIX/BNIP3 double-knockout HeLa cells.

INSTRUMENT(S): timsTOF HT

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Markus Hartl

LAB HEAD: Sascha Martens

PROVIDER: PXD060363 | Pride | 2025-01-30

REPOSITORIES: pride

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			Action	DRS
	20241126_timsHT_Adriaenssens_Martens_MPL_UniWien_MPLID24273_s1_WT_1_8c3p_GH3_1_3298.d.tar	Other
	20241126_timsHT_Adriaenssens_Martens_MPL_UniWien_MPLID24273_s2_WT_2_9c02p_GH6_1_3305.d.tar	Other
	20241126_timsHT_Adriaenssens_Martens_MPL_UniWien_MPLID24273_s3_WT_3_8c42p_GH7_1_3308.d.tar	Other
	20241126_timsHT_Adriaenssens_Martens_MPL_UniWien_MPLID24273_s4_RAPA1_1_8c59p_GH4_1_3300.d.tar	Other
	20241126_timsHT_Adriaenssens_Martens_MPL_UniWien_MPLID24273_s5_RAPA1_2_7c61p_GH5_1_3303.d.tar	Other

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