Proteomics

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TAZ (WWTR1) Interactome Analysis using a GBP nanotrap based affinity purification approach

ABSTRACT: The transcriptional co-activator TAZ (WWTR1) plays a crucial role in regulating gene expression related to cell proliferation, differentiation, and tissue homeostasis, particularly within the Hippo signaling pathway. Since TAZ does not directly bind to DNA, it largely relies on its protein-protein interaction network to perform its functions, necessitating a detailed characterization of the TAZ interactome. To achieve this, a GFP-Nanotrap-based affinity purification approach combined with LC-MS/MS protein identification was employed to document a comprehensive list of both known and novel components of the TAZ interactome in myogenic cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)

TISSUE(S): Heart, Skeletal Muscle Cell Line

SUBMITTER: John McDermott  

LAB HEAD: John Mcdermott

PROVIDER: PXD060532 | Pride | 2025-02-11

REPOSITORIES: pride

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Action DRS
20210319_UVPD_KelebeevJ_EYFPTAZ_C2C12_rep1_2.mgf Mgf
20210319_UVPD_KelebeevJ_EYFPTAZ_C2C12_rep1_2.msf Msf
20210319_UVPD_KelebeevJ_EYFPTAZ_C2C12_rep1_2.msfView Msf
20210319_UVPD_KelebeevJ_EYFPTAZ_C2C12_rep1_2.pdResult Other
20210319_UVPD_KelebeevJ_EYFPTAZ_C2C12_rep1_2.pdResultView Other
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Publications

TAZ (WWTR1) Interactome Analysis using nanotrap based affinity purification-mass spectrometry.

Kelebeev Jonathan J   MacKeracher Anastasia A   Miyake Tetsuaki T   McDermott John C JC  

Journal of cell science 20250203

Characterization of protein interactions is a fundamental goal in the post-genomic era. Here, we document a generally applicable approach to identify cellular protein interactomes using a combination of nanobody based affinity purification (A-P) coupled with tandem mass spectrometry (MS/MS). The Hippo signalling regulator TAZ functions as a transcriptional co-repressor/activator depending on its protein:protein interaction (PPI) network, we therefore undertook an unbiased proteomic screen to ide  ...[more]

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