Project description:To reveal transcriptome dynamics during adventitious root formation in a coniferous tree, C. japonica, we conducted custom microarry experiments. Three parts from cuttings of easy-to-root clone of C. japonica were collected at eight time points during adventitious root formation. The results revealed major turning points on transcriptome toward adventitious root formation and the expression behavior of genes related to carbohydrate, plant hormone and others suggested the important biological changes for adventitious root formation.
Project description:To identify carbohydrate-active enzymes in the secretome of strain A3 when L. japonica was the sole carbon source and key alginate lyases in the supernatant of fermentation broth.
Project description:Investigation of gene expression dynamics associated with male strobilus induction in Cryptomeria japonica in response to gibberellic acid (GA) treatment. [SUBMITTER_CITATION]: Transcriptome Analysis in Male Strobilus Induction by Gibberellin Treatment in Cryptomeria japonica D. Don Manabu Kurita 1,?, Kentaro Mishima 2,*,? , Miyoko Tsubomura 2, Yuya Takashima 2 , Mine Nose 2, Tomonori Hirao 2 and Makoto Takahashi 2 Forests 2020, 11(6), 633
Project description:To determine the distribution of centromere units in the genome of holocentric Chionographis japonica, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. japonica and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chio satellite repeats (16%) in the C. japonica genome before immunoprecipitation. In addition, to determine the large-scale genome organization, we performed ChIPseq by targeting the evolutionarily conserved eu- and heterochromatin-specific histone marks H3K4me2 and H33K9me2