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Oryza sativa Japonica Group

ABSTRACT: mRNA-sequence of rice mel2 mutant anthers during meiotic transition

PROVIDER: PRJDB10738 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR254130_1.fastq.gz	Fastqsanger.gz
	DRR254130_2.fastq.gz	Fastqsanger.gz
	DRR254131_1.fastq.gz	Fastqsanger.gz
	DRR254131_2.fastq.gz	Fastqsanger.gz
	DRR254132_1.fastq.gz	Fastqsanger.gz

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Oryza sativa Japonica Group

Project description:mRNA/sRNA expression analyses in rice meiotic anthers

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Oryza sativa Japonica Group transcriptome sequencing

Project description:Phosphate starvation/sufficient rice seedling, root or shoot Pi-starvation or Pi-sufficient stresses responsible rice genes, including previously unannotated genes were identified by Illumina mRNA-seq technology. 53 million reads from Pi-starvation or Pi-sufficient root or shoot tissues were uniquely mapped to the rice genome, and these included 40574 RAP3 transcripts in root and 39748 RAP3 transcripts in shoot. We compared our mRNA-seq expression data with that from Rice 44K oligomicroarray, and about 95.5% (root) and 95.4% (shoot) transcripts supported by the array were confirmed expression both by the array and by mRNA-seq, Moreover, 11888 (root) and 11098 (shoot) RAP genes which were not supported by array, were evidenced expression with mRNA-seq. Furthermore, we discovered 8590 (root) and 8193 (shoot) previously unannotated transcripts upon Pi-starvation and/or Pi-sufficient.

2012-01-06 | E-MTAB-4302 | biostudies-arrayexpress

RNA sequencing of Oryza sativa japonica control and OsRALF17/19 knockout mutant in mature rice anthers

Project description:Pollen tube growth is essential for successful fertilization and stable crop yields. We constructed loss-of-function/knock-out mutants that simultaneously target two rice genes using the CRISPR/Cas9 mutagenesis system. The selected OsRALF17 and OsRALF19 genes are strongly expressed in rice bicellular/tricellular pollen and have essential functions in the pollen tube growth. For the corresponding transcriptomic analysis, we sampled mature pollen anthers from a control group and an OsRALF17/19 knock-out mutant.

2023-09-29 | E-MTAB-12295 | biostudies-arrayexpress

Oryza sativa Japonica Group

Project description:The transcriptome of anthers of rice gamyb mutant expressing various GAMYB homologs was measured at the microspore stage.

| PRJNA146963 | ENA

RNA sequencing of control and OsADF1/6/9 triple knockout mutant in mature rice anthers (Oryza sativa japonica)

Project description:We created a triple loss-of-function/knockout mutant targeting three rice genes simultaneously. The three selected genes are as follows: OsADF1 (LOC_Os02g44470), OsADF6 (LOC_Os04g46910), and OsADF9 (LOC_Os07g30090). These three ADFs are strongly transcriptional expressed in the rice mature anthers (stages 13) and bi-/tricelluler pollen. The triple mutant of these OsADFs does not produce self-fertilizing seeds due to the short length of the pollen tube (male-sterile). This data is about mature anther transcriptome data about the triple mutant of OsADFs (ADFmT). We sampled mature anther for the analysis.

2024-07-02 | E-MTAB-13557 | biostudies-arrayexpress

Oryza sativa Japonica Group

Project description:Transcriptome analysis in rice smos1 mutant

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RNA-Seq of Oryza sativa Dongjin (rice) wild type and gori mutant anthers

Project description:Rice anthers at anthesis stage from the wild type and gori mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

2020-11-03 | E-MTAB-9726 | biostudies-arrayexpress

Oryza sativa Japonica Group

Project description:MutMap+: Genetic mapping and mutant identification without crossing in rice

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Rice plants (Oryza sativa var. japonica): WT vs. MIT knockdown plant

Project description:Transcriptional profiling of MIT knockdown plants. MIT is a mitochondrial Fe transporter essential for rice growth and development. The goal was to determine the effects of MIT on global rice gene expression.

2011-04-07 | GSE28428 | GEO

Oryza sativa japonica Transcriptome or Gene expression

Project description:In this study, we examined the transcriptome dynamics within the matured fully expanded rice leaf and used strand-specific RNA sequencing to generate a comprehensive transcriptome dataset for the mature rice leaf. The rice Nipponbare (Oryza sativa l. japonica) seedlings were grown in the greenhouse. About 20 days after planting, the fully opened 4th leaves was cut it into seven 3-cm segments, from bottom to tip and labeled as sections 1 to 7, respectively. The tissues were immediately frozen in liquid nitrogen for total RNA extraction. Two biological replicates were collected for each section. Note: All samples in SRA were assigned the same sample accession (SRS685294). This is incorrect as there are different samples, hence âSource Nameâ was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

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