Project description:To find epigenetic factors potentially involved in Sorafenib resistance in hepatocellular carcinoma cells, an epigenetic factors-targeted CRISPR/Cas9 library was applied to perform the screening.
Project description:To acquire an overall picture of IMP1 regulating network, we compared the global transcription pattern in IMP1 inhibited JAR cells with control cells by DNA microarray. Specific siRNA to IMP1 or universal negtive control siRNA were transfected into JAR cells respectively, the total RNA was extracted after 72hr incubation. Then went into the microarray assay procedure.
Project description:Genome-wide CRISPR-Cas9 knockout screen using TKOv1 sgRNA library performed in isogenic RBM10-proficient and RBM10-deficient HCC827 cells.
Project description:Genome-wide CRISPR-Cas9 knockout screen using TKOv1 sgRNA library was performed in isogenic RBM10-proficient and RBM10-deficient HCC827 cells.
