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Staphylococcus aureus

ABSTRACT: Whole genome sequence of Staphylococcus aureus isolated in the intensive care unit of a tertiary medical hospital in Japan

PROVIDER: PRJDB13532 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR375640_1.fastq.gz	Fastqsanger.gz
	DRR375640_2.fastq.gz	Fastqsanger.gz
	DRR375641_1.fastq.gz	Fastqsanger.gz
	DRR375641_2.fastq.gz	Fastqsanger.gz
	DRR375642_1.fastq.gz	Fastqsanger.gz

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intensive care unit

Project description:intensive care unit Raw sequence reads

| PRJNA561526 | ENA

Critically ill patients with either gram-positive sepsis or gram-negative sepsis

Project description:Objective: It is unclear whether the host response of gram-positive sepsis differs from gram-negative sepsis at a transcriptome level. Using microarray technology, we compared the gene-expression profiles of gram-positive sepsis and gram-negative sepsis in critically ill patients. Design: A prospective cross-sectional study. Setting: A 20-bed general intensive care unit of a tertiary referral hospital. Patients: Seventy-two patients admitted to the intensive care unit. Interventions: Intravenous blood was collected for leukocyte separation and RNA extraction. Microarray experiements were then performed examing the expression level of 19,232 genes in each sample. Measurements and Main Results: There was no difference in the expression profile between gram-positive and gram-negative sepsis. The finding remained unchanged even when genes with lower expression level were included or after statistical stringency was lowered. There were, however, ninety-four genes differentially expressed between sepsis and control patients. These genes included those involved in immune regulation, inflammation and mitochondrial function. Hierarchical cluster analysis confirmed that the difference in gene expression profile existed between sepsis and control patients, but not between gram-positive and gram-negative patients. Conclusion: Gram-positive and gram-negative sepsis share a common host response at a transcriptome level. These findings support the hypothesis that the septic response is non-specific and is designed to provide a more general response that can be elicited by a wide range of different micro-organisms. Keywords: disease state analysis, gram-positive sepsis, gram-negative sepsis

2007-12-01 | GSE6535 | GEO

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