Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Plastisphere metagenome

ABSTRACT: Investigation of the degree of degradation and attached microbiome of biodegradable plastics submerged in the sea

PROVIDER: PRJDB14280 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR403672_1.fastq.gz	Fastqsanger.gz
	DRR403672_2.fastq.gz	Fastqsanger.gz
	DRR403673_1.fastq.gz	Fastqsanger.gz
	DRR403673_2.fastq.gz	Fastqsanger.gz
	DRR403674_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 202

Similar Datasets

Plastisphere microbiome of Biodegradable Plastics on deep-sea floor

Project description:Plastisphere microbiome of Biodegradable Plastics on deep-sea floor

| PRJNA886482 | ENA

Plastics investigated can be mineralized by bacteria naturally occurring within the marine environment

Project description:Biodegradable plastics are one possible solution for reducing plastic waste, yet the mechanisms and organisms involved in their degradation in the aquatic environment remain understudied. In this study, we have enriched a microbial community from North Sea water and sediment, capable of growing on the polyester poly(butylene succinate). This culture was grown on two other biodegradable polyesters, polycaprolactone and ecovio® FT (a PBAT-based blended biodegradable plastic), and the differences between community structure and activity on these three polymers were determined by metagenomics and metaproteomics. We have seen that the plastic supplied drives the community structure and activity. Setups growing on ecovio® FT were more diverse, yet showed the lowest degradation, while poly(butylene succinate) and polycaprolactone resulted in a less diverse community but much higher degradation efficiencies. The dominating species were Alcanivorax sp., Thalassobius sp., or Pseudomonas sp., depending on the polymer supplied. Furthermore, we have observed that Gammaproteobacteria were more abundant and active within the biofilm and Alphaproteobacteria within the free-living fraction of the enrichments. Two of the three PETase-like enzymes isolated were expressed as tandems (Ple -tan1 &Ple – tan2) and all three were produced by Pseudomonas sp. Of those, Ple-tan1 was most active on all three substrates and also the most thermostable. Overall, we could show that all three plastics investigated can be mineralized by bacteria naturally occurring within the marine environment and characterize some of the enzymes involved in the degradation process.

2023-11-22 | PXD038098 | Pride

plastisphere in lakes

Project description:Microbial colonization patterns and biodegradation of petrochemical and biodegradable plastics in lake waters: insights from a field experiment

| PRJNA905066 | ENA

Moesziomyces antarcticus

Project description:Fungus with a strong degradation activity for biodegradable plastics

| PRJNA186884 | ENA

Project description:Microbial community on biodegradable plastics

| PRJNA985132 | ENA

Multiomics study to elucidate the biodegradation of copolyester film by a marine microbial enrichment culture

Project description:Although the biodegradation of biodegradable plastics in soil and compost is well-studied, there is little knowledge on the metabolic mechanisms of synthetic polymers degradation by marine microorganisms. Here, we present a multiomics study to elucidate the biodegradation mechanism of a commercial aromatic-aliphatic copolyester film by a marine microbial enrichment culture. The plastic film and each monomer can be used as sole carbon source. Our analysis showed that the consortium synergistically degrades the polymer, different degradation steps being performed by different members of the community. Analysis of gene expression and translation profiles revealed that the relevant degradation processes in the marine consortium are closely related to poly(ethylene terephthalate) biodegradation from terrestrial microbes. Although there are multiple genes and organisms with the potential to perform a degradation step, only a few of these are active during biodegradation. Our results elucidate the potential of marine microorganisms to mineralize biodegradable plastic polymers and describe the mechanisms of labor division within the community to get maximum energetic yield from a complex synthetic substrate.

2020-09-25 | PXD018391 | Pride

Plastisphere Metagenome

Project description:Bacterial biofilms colonizing plastics and microplastics in estuarine waters, with an emphasis on Vibrio spp. and their antibacterial resistance

| PRJNA645698 | ENA

Bacteria

Project description:Collection of marine microorganisms that degradate biodegradable plastics

| PRJDB17481 | ENA

RNA-Seq of shallow-water mussel Mytilus galloprovincialis under in situ hydrostatic pressure exposure

Project description:Recent studies have unveiled the deep sea as a rich biosphere, populated by species descended from shallow-water ancestors post-mass extinctions. Research on genomic evolution and microbial symbiosis has shed light on how these species thrive in extreme deep-sea conditions. However, early adaptation stages, particularly the roles of conserved genes and symbiotic microbes, remain inadequately understood. This study examined transcriptomic and microbiome changes in shallow-water mussels Mytilus galloprovincialis exposed to deep-sea conditions at the Site-F cold seep in the South China Sea. Results reveal complex gene expression adjustments in stress response, immune defense, homeostasis, and energy metabolism pathways during adaptation. After 10 days of deep-sea exposure, shallow-water mussels and their microbial communities closely resembled those of native deep-sea mussels, demonstrating host and microbiome convergence in response to adaptive shifts. Notably, methanotrophic bacteria, key symbionts in native deep-sea mussels, emerged as a dominant group in the exposed mussels. Host genes involved in immune recognition and endocytosis correlated significantly with the abundance of these bacteria. Overall, our analyses provide insights into adaptive transcriptional regulation and microbiome dynamics of mussels in deep-sea environments, highlighting the roles of conserved genes and microbial community shifts in adapting to extreme environments.

2024-11-24 | GSE263620 | GEO

Project description:Microbial degradation of polyethylene plastics

| PRJNA936653 | ENA