Project description:BackgroundEntamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation.Methodology/principal findingsWe sequenced a small RNA library prepared from a culture of trophozoites of Entamoeba histolytica Strain HM1-IMSS using a deep DNA sequencing approach. Deep sequencing yielded 16 million high-quality short sequence reads containing a total of 5 million non-redundant sequence reads. Based on a bioinformatics pipeline, we found that only 0.5% of these non-redundant small RNA reads were a perfect match with the drafted E. histolytica genome. We did not find miRNA homologs in plant or animal miRNAs. We discovered 199 new potential Entamoeba histolytica miRNAs. The expression and sequence of these Ehi-miRNAs were further validated through microarray by µParaflo Microfluidic Biochip Technology. Ten potential miRNAs were additionally confirmed by real time RT-PCR analysis. Prediction of target genes matched 32 known genes and 34 hypothetical genes.Conclusions/significanceThese results show that there is a number of regulatory miRNAs in Entamoeba histolytica. The collection of miRNAs in this parasite could be used as a new platform to study genomic structure, gene regulation and networks, development, and host-parasite interactions.

| S-EPMC3709888 | biostudies-literature

Entamoeba histolytica HM-3:IMSS

Project description:Entamoeba histolytica HM-3:IMSS Genome sequencing and assembly

| PRJNA72935 | ENA

Entamoeba histolytica HM-1:IMSS-B

Project description:Entamoeba histolytica HM3:IMSS-B genome sequencing project

| PRJNA51239 | ENA

Entamoeba histolytica KU48

Project description:Entamoeba histolytica KU48

| PRJNA51235 | ENA

Gene expression in phagocytic and non-phagocytic Entamoeba histolytica populations

Project description:The ability of Entamoeba histolytica to phagocytose host cells correlates to observed virulence in vivo. To better understand the mechanism of phagocytosis we used paramagnetic beads coated with host ligand and sorted trophozoites based on phagocytic ability. Gene expression was then measured in both the sorted phagocytic and non-phagocytic populations using a custom Affymetrix chip for E. histolytica. Feed forward regulation of phagocytosis by Entamoeba histolytica. Infection and Immunity. PMID 23045476

2012-10-16 | GSE41604 | GEO

Entamoeba histolytica HM-1:IMSS-A

Project description:Entamoeba histolytica HM-1:IMSS-A Genome sequencing and assembly

| PRJNA51237 | ENA