Project description:Syrian golden hamsters were treated with TIP or control RNA and infected with SARS-CoV2, at 5 days post infection, RNA was extracted and RNAseq was performed, uninfected hamsters treated with TIP or Control RNA were used as controls.
Project description:Changes in the gene expression in the inferior colliculus of the sound stimulated both control Syrian hamsters and the strain GASH:Sal.
Project description:Hyperlipidemia is one of high risk factors for cardiovascular disease (CVD), whose regulation is known to be mediated also by microRNA-mediated mechanisms. Therefore, the analysis of transcriptomics data would gain insights into the molecular mechanisms implicating microRNAs (miRNAs) behind the atherogenic hyperlipidemia. The goal of our study was to profile the specific miRNAs expression associated with atherogenic hyperlipidemia. To this aim, we performed miRNAs microarray analysis for different tissues (liver, left ventricle of the heart, small intestine and blood mononuclear cells) obtained from the experimental model of high-fat diet induced hyperlipidemic hamsters (Mesocricetus auratus). The microarray data were obtained using miRCURY LNA microRNA arrays 7 th generation platform with miRBase 19 (Exiqon, Denmark), using total RNA isolated from hyperlipidemic (HL) and normolipidemic (NL) hamsters? tissues and blood cells. The animals were fed for 12 weeks either standard rodent chow supplemented with 3% cholesterol and 15% unsalted butter (HL) or standard rodent chow (NL), with free access to food and water.
Project description:Using microarray analyses and subsequent verification by RT-PCR, we studied the changes in gene expression in the inferior colliculus after an ictal event in one models of audiogenic epilepsy, genetic audiogenic seizure hamster (GASH:Sal). GASH:Sal, a hamster strain developed at the University of Salamanca, exhibits genetic audiogenic epilepsy similar to human Grand Mal epilepsy. GASH:Sal shows an autosomal recessive inheritance for susceptibility to audiogenic seizures, which manifest more severely in young animals; the seizure severity progressively declines with age. Genetic animal models of epilepsy are an important tool for further understanding the basic cellular mechanisms underlying epileptogenesis and for developing novel antiepileptic drugs. We conducted a comparative study of gene expression in the inferior colliculus, a nucleus that triggers audiogenic seizures, using two animal models, the Wistar audiogenic rat (WAR) and the genetic audiogenic seizure hamster (GASH:Sal). For this purpose, both models were subjected to auditory stimulation, and 60 minutes after stimulation, the inferior colliculi were collected. As a control, intact Wistar rats and Syrian hamsters were subjected to identical stimulation and tissue preparation protocols to those performed on the experimental animals. A total of 24 animals were used in this study according to the following distribution: 12 control Syrian hamsters (Mesocricetus auratus) and 12 GASH:Sal at 16 weeks of age and a body weight of approximately 60 g. Six animals Syrian and GASH:Sal hamsters, respectively, were exposed to auditory stimulation, and 60 min after the seizures, we harvested the IC for all gene expression analyses (stimulated Syrian hamsters and stimulated GASH:Sal hamsters). As controls, other six animals Syrian and GASH:Sal hamsters, respectively, were not exposed to the same stimulation (Syrian hamsters and GASH:Sal hamsters).