Pig gut metagenome
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ABSTRACT:
PROVIDER: PRJDB14476 | ENA |
REPOSITORIES: ENA
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Project description:Oral administration of an extract of compost fermented with thermophiles to pigs reduces the incidence of stillbirth and promotes piglet growth. However, the mechanism by which compost extract modulates the physiological conditions of the animals remains largely unknown. Here, we investigate the effects of compost extract on the gene expression in the intestine of the rat as a mammalian model. Gene expression analyses of the intestine indicated that several immune-related genes were upregulated following compost exposure. Thus, thermophile-fermented compost can contain microbes and/or substances that activate the gut mucosal immune response in the rat.
2012-11-02 | GSE37732 | GEO
Project description:Oral administration of an extract of compost fermented with thermophiles to pigs reduces the incidence of stillbirth and promotes piglet growth. However, the mechanism by which compost extract modulates the physiological conditions of the animals remains largely unknown. Here, we investigate the effects of compost extract on the gene expression in the intestine of the rat as a mammalian model. Gene expression analyses of the intestine indicated that several immune-related genes were upregulated following compost exposure. Thus, thermophile-fermented compost can contain microbes and/or substances that activate the gut mucosal immune response in the rat. In Male Wistar rats aged 3 weeks, tap water was supplemented with 1.0% (v/v) compost extract for the experimental rats, whereas water only was given to the control rats. The rats received water ad libitum for 12 weeks. Fresh gut samples were collected from individual rats at the end of the feeding test and stored at -80°C. The intestine were separated from the gut and used as samples for the isolation of total RNA. otal RNA was then subjected to microarray experiments using the Whole Rat Genome (4x44k) Oligo Microarray (Agilent Technologies, Inc.)
2012-11-02 | E-GEOD-37732 | biostudies-arrayexpress
Project description:In this study, BS-seq technology was used to analyze the epigenomic analysis of liver tissues of Rongchang pigs at three important feeding stages. There was little difference in DNA methylation profiles among the neonatal, weaned and adult groups. However, we observed significant changes in DNA methylation in the gene body and promoter regions of metabolic and immune-related genes from neonates to adulthood. Correlation analysis showed that the methylation level of gene promoters was significantly negatively correlated with gene expression. In neonatal Rongchang pigs, most genes related to nutritional metabolism lost DNA methylation and mRNA expression increased. The few fatty acid metabolism-related genes that were hypomethylated and highly expressed in the early development of Rongchang pigs may satisfy the nutritional requirements of Rongchang pig neonates, and their immune-related genes may be related to the establishment of liver immunity.
2024-09-27 | GSE266050 | GEO
Project description:Multi-omics analyses of metabolites and rhizobacteria of carrot fertilized with thermophile-fermented compost
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Project description:In this study, the SAF and VAF development was characterized and compared in SAF and VAF of Bama pig during postnatal development (infancy, puberty and adulthood) using RNA sequencing technologies (RNA-Seq). We profiled the transcriptome of SAF and VAF isolated from 1-, 3- and 6-month-old pigs and identified 23636 expressed transcription units. Of the them, 1165 genes were differentially expressed between the despots and /or developmental stages. Upregulated gene in SAF of Bama pigs showed significant function and pathway enrichment in central nervous system development, lipid metabolism, oxidation-reduction process, cell adhesion while genes involved in immune system process, actin cytoskeleton organization, male gonad development, hippo signaling pathway preferentially expressed in VAF. Short time-series expression miner analysis demonstrated the differentiation in gene expression patterns between the two deposit was corresponding to their distinct responses in sextual development, hormone signaling pathway, lipid metabolism as well as hippo signaling pathway. Transcriptome analysis of SAF and VAF in Bama pigs suggested that the depot differences of the adipose tissue are not only related to lipid metabolism and endocrine function, but also closely associated with sextual development and organ size regulation.
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2023-07-17 | GSE237153 | GEO
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2024-01-01 | GSE233410 | GEO
Project description:In summary, we have for the first time generated a Dox inducible and switchable tdTomato pig strain with dual knock-in at Hipp11 and Rosa26 locus. This pig model provide a versatile tool model to produce stable transgenic pigs with inducible overexpressing any gene of interest by using the technology of phiC31 integrase-mediated cassette exchange. Based on this approach, an inducible hKRASG12D-expressing pig model line was established and tumor formation was induced after Dox administration. We expect that the inducible tool pig model would greatly facilitate the production of controllable transgenic pigs, and broaden the applications of transgenic pigs in biomedicine and agriculture fields.
2021-06-10 | GSE176519 | GEO