Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Leptothrix cholodnii SP-6

ABSTRACT: Determination of mutation points in a sheath-less mutant of Leptothrix cholodnii SP-6

PROVIDER: PRJDB14526 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR412192_1.fastq.gz	Fastqsanger.gz
	DRR412192_2.fastq.gz	Fastqsanger.gz
	DRR412193_1.fastq.gz	Fastqsanger.gz
	DRR412193_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Leptothrix cholodnii

Project description:Leptothrix cholodnii overview

| PRJNA36227 | ENA

Leptothrix cholodnii SP-6

Project description:RNA sequencing of wild-type and lthC mutant in Leptothrix cholodnii SP-6

| PRJDB18168 | ENA

Leptothrix cholodnii SP-6

Project description:DNA sequencing of wild-type and lthC mutant in Leptothrix cholodnii SP-6

| PRJDB18186 | ENA

<i>Leptothrix cholodnii</i> Response to Nutrient Limitation.

Project description:Microorganisms are widely utilized for the treatment of wastewater in activated sludge systems. However, the uncontrolled growth of filamentous bacteria leads to bulking and adversely affects wastewater treatment efficiency. To clarify the nutrient requirements for filament formation, we track the growth of a filamentous bacterium, Leptothrix cholodnii SP-6 in different nutrient-limited conditions using a high aspect-ratio microfluidic chamber to follow cell-chain elongation and sheath formation. We find that limitations in Na+, K+, and Fe2+ yield no observable changes in the elongation of cell chains and sheath formation, whereas limitations of C, N, P, or vitamins lead to more pronounced changes in filament morphology; here we observe the appearance of partially empty filaments with wide intercellular gaps. We observe more dramatic differences when SP-6 cells are transferred to media lacking Mg2+ and Ca2+. Loss of Mg2+ results in cell autolysis, while removal of Ca2+ results in the catastrophic disintegration of the filaments. By simultaneously limiting both carbon and Ca2+ sources, we are able to stimulate planktonic cell generation. These findings paint a detailed picture of the ecophysiology of Leptothrix, which may lead to improved control over the unchecked growth of deleterious filamentous bacteria in water purification systems.

| S-EPMC8264430 | biostudies-literature

Leptothrix sp. C29

Project description:Leptothrix sp. C29 Genome sequencing and assembly

| PRJNA632164 | ENA

Leptothrix ochracea L12

Project description:Leptothrix ochracea L12 Genome sequencing project

| PRJNA56115 | ENA

Leptothrix cholodnii SP-6

Project description:Iron-oxidizing freshwater bacterium

| PRJNA20039 | ENA

Development of a Gene Replacement Method for the Filamentous Bacterium<i>Leptothrix cholodnii</i>SP-6.

Project description:Genetic strategies such as gene disruption and fluorescent protein tagging largely contribute to understanding the molecular mechanisms of biological functions in bacteria. However, the methods for gene replacement remain underdeveloped for the filamentous bacteriaLeptothrix cholodniiSP-6. Their cell chains are encased in sheath composed of entangled nanofibrils, which may prevent the conjugation for gene transfer. Here, we describe a protocol optimized for gene disruption through gene transfer mediated by conjugation withEscherichia coliS17-1 with details on cell ratio, sheath removal, and loci validation. The obtained deletion mutants for specific genes can be used to clarify the biological functions of the proteins encoded by the target genes. Graphical overview.

| S-EPMC10127049 | biostudies-literature

Leptothrix discophora strain:CCM 2812

Project description:Leptothrix discophora strain:CCM 2812 Genome sequencing and assembly

| PRJNA1002076 | ENA

Genome-wide gene expression profiling of rice Zhonghua11 and ila1 lamina joint

Project description:The leaf lamina joint joins the rice leaf blade and sheath, contributing significantly to the leaf angle trait. A more erect leaf facilitates the penetration of sunlight, enhancing photosynthetic efficiency and occupying less space in dense planting. Genetic screening found a mutant increased leaf angle1, ila1 from rice T-DNA insertional mutants library. We used microarrays to detail the transcriptional profile changes in the mutant ila1 lamina joint.

2012-05-01 | GSE33361 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data