Bombyx mori strain:T04 x T04
Ontology highlight
ABSTRACT:
PROVIDER: PRJDB14974 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DRR425961_1.fastq.gz | Fastqsanger.gz | |||
| DRR425961_2.fastq.gz | Fastqsanger.gz | |||
| DRR425962_1.fastq.gz | Fastqsanger.gz | |||
| DRR425962_2.fastq.gz | Fastqsanger.gz | |||
| DRR425963_1.fastq.gz | Fastqsanger.gz |
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Project description:Transcriptome analysis of the silkworm m04 lethal (l-m04) mutant
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Project description:Background: MicroRNA (miRNA) and other small regulatory RNAs contribute to the modulation of a large number of cellular processes. We sequenced three total RNA libraries prepared from the whole body, and the anterior and posterior silk glands of Bombyx mori, with a view to expanding the repertoire of silkworm miRNAs and exploring transcriptional differences in miRNAs between segments of the silk gland. Results: With the aid of large-scale Solexa sequencing technology, we validated 244 unique miRNA genes, including 191 novel and 53 previously reported genes, corresponding to 309 loci in the silkworm genome. Interestingly, 24 unique miRNAs were widely conserved from invertebrates to vertebrates; 12 unique ones were limited to invertebrates and 33 were confined to insects; whereas the majority of the newly identified miRNAs were silkworm-specific. We identified 21 clusters and 42 paralogs of miRNAs in the silkworm genome. However, sequence tags showed that paralogs or clusters are not prerequisites for coordinated transcription and accumulation. The majority of silkworm-specific miRNAs are located in transposable elements, and display significant differences in abundance between the anterior and posterior silk glands. Conclusions: Conservative analysis revealed that miRNAs serve as phylogenetic markers and function in evolutionary signaling. The newly identified miRNAs greatly enriched the repertoire of insect miRNAs, and provide insights into miRNA evolution, biogenesis, and expression in insects. The differential expression of miRNAs in the anterior and posterior silk glands supports their involvement as new layers in the regulation of the silkworm silk gland.
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Project description:Transcriptome analysis of the epidermis of the purple quail-like (q-lp) mutant of silkworm, Bombyx mori
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Project description:Background: MicroRNA (miRNA) and other small regulatory RNAs contribute to the modulation of a large number of cellular processes. We sequenced three total RNA libraries prepared from the whole body, and the anterior and posterior silk glands of Bombyx mori, with a view to expanding the repertoire of silkworm miRNAs and exploring transcriptional differences in miRNAs between segments of the silk gland. Results: With the aid of large-scale Solexa sequencing technology, we validated 244 unique miRNA genes, including 191 novel and 53 previously reported genes, corresponding to 309 loci in the silkworm genome. Interestingly, 24 unique miRNAs were widely conserved from invertebrates to vertebrates; 12 unique ones were limited to invertebrates and 33 were confined to insects; whereas the majority of the newly identified miRNAs were silkworm-specific. We identified 21 clusters and 42 paralogs of miRNAs in the silkworm genome. However, sequence tags showed that paralogs or clusters are not prerequisites for coordinated transcription and accumulation. The majority of silkworm-specific miRNAs are located in transposable elements, and display significant differences in abundance between the anterior and posterior silk glands. Conclusions: Conservative analysis revealed that miRNAs serve as phylogenetic markers and function in evolutionary signaling. The newly identified miRNAs greatly enriched the repertoire of insect miRNAs, and provide insights into miRNA evolution, biogenesis, and expression in insects. The differential expression of miRNAs in the anterior and posterior silk glands supports their involvement as new layers in the regulation of the silkworm silk gland. Sequencing three total RNA pools of the whole silkworm body from 5th-instar day-3 larvae, and anterior and posterior silkworm silk glands, using the latest sequencing Solexa technology
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Project description:Transcriptome analysis of silkworm midgut during the fourth molting stage
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Project description:Maternal genes present in mature oocytes and play a crucial role in the early development of the silkworm. Although maternal genes have been widely studied in many species, there are limited research in Bombyx mori. High-through experimental technology provides a practical way for genome-wide study. Here we show a transcriptome study on silkworm eggs. Unfertilized eggs from five different stages of early development were used for detecting the change situation of gene expression. The expressed genes showed different patterns over time. Maternal genes were annotated according to homology analysis with Drosophila melanogaster. In our result, more than a half expressed maternal genes were fall into four expression patterns and showed downward trend over time. The function annotation of material genes showed that they were related to transcription factor activity, growth factor activity, nucleic acid binding, RNA binding, ATP binding, ion binding and so on. In additional, twenty-two gene clusters including material genes were identified from 18 scaffolds. Altogether, we plotted a profile for maternal genes of Bombyx mori using digital gene expression profiling method. It will provide a base for mother-specific signature research and improve the understanding of early development of silkworm.
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