Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Genomic localization of FLAG-tagged WH1 of MOZ and MORF in HEK293T cells

PROVIDER: PRJDB14992 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR426258_1.fastq.gz	Fastqsanger.gz
	DRR426258_2.fastq.gz	Fastqsanger.gz
	DRR426259_1.fastq.gz	Fastqsanger.gz
	DRR426259_2.fastq.gz	Fastqsanger.gz
	DRR426260_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

MORF and MOZ acetyltransferases target unmethylated CpG islands through the winged helix domain

Project description:Human acetyltransferases MOZ and MORF are implicated in chromosomal translocations associated with aggressive leukemias. Oncogenic translocations involve the far amino terminus of MOZ/MORF, the function of which remains unclear. Here, we identified and characterized two structured winged helix domains, WH1 and WH2, in MORF and MOZ. WHs bind DNA in a cooperative manner, with WH1 specifically recognizing unmethylated CpG sequences. Structural and genomic analyses show that the DNA binding function of WHs targets MORF/MOZ to gene promoters, stimulating transcription and H3K23 acetylation, and WH1 recruits oncogenic fusions to HOXA genes that trigger leukemogenesis. Cryo-EM, NMR, mass spectrometry and mutagenesis studies provide mechanistic insight into the DNA-binding mechanism, which includes the association of WH1 with the linker DNA and binding of WH2 to the dyad of the nucleosome, with the latter being further modulated by the neighboring domain DPF and autoacetylation of MORF. The discovery of WHs in MORF and MOZ and their DNA binding functions could open a new avenue in developing therapeutics to treat diseases associated with aberrant MOZ/MORF acetyltransferase activities.

2023-03-11 | PXD036192 | Pride

Homo sapiens

Project description:Genomic localization of MOZ mutant proteins in HEK293T cells

| PRJDB13722 | ENA

Homo sapiens

Project description:Genomic localization of FLAG-tagged PHF16 and its PZP domain-deletion mutant in HEK293T cells

| PRJDB15670 | ENA

ChIP-seq of MOZ (Flag-V5-BIO tagged) in mouse mouse craniofacial development

Project description:Carboxy-terminally tagged MOZ (Flag-V5-BIO tagged) was detected by ChIP-seq using anti-V5 antibody (Sigma, A7345) to precipitate chromatin associated with MOZ

2019-07-23 | GSE134607 | GEO

Homo sapiens

Project description:Genomic localization of ENL and DOT1L in HEK293T cells deficient for MOZ

| PRJDB14995 | ENA

Mapping cell structure across scales by fusing protein images and interactions

Project description:Enhanced crosslinking and immunoprecipitation assay (eCLIP) for C-terminally FLAG-HA-tagged RPS3A in HEK293T cells.

2021-08-23 | GSE171553 | GEO

Homo sapiens

Project description:Genomic localization of H3K14ac marks in HEK293T cells

| PRJDB13723 | ENA

RANK ligand converts the NCoR/HDAC3 co-repressor to a PGC1β- and RNA-dependent co-activator of osteoclast gene expression [ChIP-seq]

Project description:To investigate the genomic localization of NCoR/HDAC3/PGC1β complex and the enhancer/promoter activity in the regulation of osteoclast differentiation, we used NCoR KO or PGC1β KO bone marrow cells, non-coding RNA Dancr/Rnu12 siRNA knockdown bone marrow cells and FLAG-tagged PGC1β RNA recognition motif deletion mutant expressed RAW 264.7 cells. We then performed chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H3K27ac, NCoR, HDAC3, PGC1β, p65, Fosl2, PU.1 and FLAG-tag.

2022-08-22 | GSE211672 | GEO

AF9/MLLT3 ChIP-Seq in LPS-stimulated RAW264.7 cells

Project description:We sought to characterize the genomic localization of the YEATS domain protein AF9 in the macrophage-like cell line RAW 264.7 +/- LPS stimulation and +/- sodium crotonate pretreatment. We performed chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-seq) for endogenous AF9 or for a FLAG-tagged AF9 transgene in RAW264.7 or a RAW264.7 cell line engineered to express FLAG-AF9, respectively.

2016-04-21 | GSE76539 | GEO

Localization of wildtype Ioc4 in wildtype and set2 yeast strains. Localization of Ioc4 mutants (dPWWP, 2KE, dINS) in wildtype yeast strains.

Project description:ChIP-chip assays to assess the localization of FLAG-tagged, wildtype and mutant Ioc4 in yeast.

2022-01-24 | GSE165984 | GEO