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Ralstonia pseudosolanacearum strain:OE1-1

ABSTRACT: Transcriptome analysis of plant cell wall degradation enzyme coding gene

PROVIDER: PRJDB15155 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR438005_1.fastq.gz	Fastqsanger.gz
	DRR438005_2.fastq.gz	Fastqsanger.gz
	DRR438006_1.fastq.gz	Fastqsanger.gz
	DRR438006_2.fastq.gz	Fastqsanger.gz
	DRR438007_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of quorum sensing genes in Ralstonia pseudosolanacerum strain OE1-1

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Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of siderophore production-related genes in Ralstonia pseudosolanacerum strain OE1-1

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Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of downstream genes of quorum sensing in Ralstonia pseudosolanacerum strain OE1-1

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Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of signal transduction pathway in quorum sensing in Ralstonia pseudosolanacerum strain OE1-1

| PRJDB16932 | ENA

The transcription regulator ChpA affects the global transcriptome including quorum sensing-dependent genes in Ralstonia pseudosolanacearum strain OE1-1.

Project description:The gram-negative plant-pathogenic β-proteobacterium Ralstonia pseudosolanacearum strain OE1-1 produces methyl 3-hydroxymyristate as a quorum sensing (QS) signal through methyltransferase PhcB and senses the chemical via the sensor histidine kinase PhcS. This leads to activation of the LysR family transcription regulator PhcA, which regulates the genes (QS-dependent genes) responsible for QS-dependent phenotypes, including virulence. The transcription regulator ChpA, which possesses a response regulator receiver domain and also a hybrid sensor histidine kinase/response regulator phosphore-acceptor domain but lacks a DNA-binding domain, is reportedly involved in QS-dependent biofilm formation and virulence of R. pseudosolanacearum strain GMI1000. To explore the function of ChpA in QS of OE1-1, we generated a chpA-deletion mutant (ΔchpA) and revealed that the chpA deletion leads to significantly altered QS-dependent phenotypes. Furthermore, ΔchpA exhibited a loss in its infectivity in xylem vessels of tomato plant roots, losing virulence on tomato plants, similar to the phcA-deletion mutant (ΔphcA). Transcriptome analysis showed that the transcript levels of phcB, phcQ, phcR, and phcA in ΔchpA were comparable to those in OE1-1. However, the transcript levels of 89.9% and 88.9% of positively and negatively QS-dependent genes, respectively, were significantly altered in ΔchpA compared with OE1-1. Furthermore, the transcript levels of these genes in ΔchpA were positively correlated with those in ΔphcA. Together, our results suggest that ChpA is involved in the regulation of these QS-dependent genes, thereby contributing to the behaviour in host plant roots and virulence of OE1-1.

| S-EPMC10576176 | biostudies-literature

PhcQ mainly contributes to the regulation of quorum sensing-dependent genes, in which PhcR is partially involved, in Ralstonia pseudosolanacearum strain OE1-1.

Project description:The gram-negative plant-pathogenic β-proteobacterium Ralstonia pseudosolanacearum strain OE1-1 produces methyl 3-hydroxymyristate as a quorum sensing (QS) signal via the methyltransferase PhcB and senses the chemical through the sensor histidine kinase PhcS. This leads to functionalization of the LysR family transcriptional regulator PhcA, regulating QS-dependent genes responsible for the QS-dependent phenotypes including virulence. The phc operon consists of phcB, phcS, phcR, and phcQ, with the latter two encoding regulator proteins with a receiver domain and a histidine kinase domain and with a receiver domain, respectively. To elucidate the function of PhcR and PhcQ in the regulation of QS-dependent genes, we generated phcR-deletion and phcQ-deletion mutants. Though the QS-dependent phenotypes of the phcR-deletion mutant were largely unchanged, deletion of phcQ led to a significant change in the QS-dependent phenotypes. Transcriptome analysis coupled with quantitative reverse transcription-PCR and RNA-sequencing revealed that phcB, phcK, and phcA in the phcR-deletion and phcQ-deletion mutants were expressed at similar levels as in strain OE1-1. Compared with strain OE1-1, expression of 22.9% and 26.4% of positively and negatively QS-dependent genes, respectively, was significantly altered in the phcR-deletion mutant. However, expression of 96.8% and 66.9% of positively and negatively QS-dependent genes, respectively, was significantly altered in the phcQ-deletion mutant. Furthermore, a strong positive correlation of expression of these QS-dependent genes was observed between the phcQ-deletion and phcA-deletion mutants. Our results indicate that PhcQ mainly contributes to the regulation of QS-dependent genes, in which PhcR is partially involved.

| S-EPMC8578825 | biostudies-literature

Impact of pehR transcriptional regulator on Ralstonia solanacearum F1C1 gene expression patterns

Project description:Ralstonia solanacearum, recognized as a pervasive plant pathogen causing lethal wilt disease in over 450 plant species worldwide, represents a substantial threat to economically vital crops including tomato, chili, and eggplant. Within R. solanacearum, pehR serves as a transcriptional regulator, forming part of the pehSR two-component regulatory system. This system governs the production of polygalacturonase (PG), a crucial extracellular enzyme involved in plant cell wall degradation and bacterial wilt development. Our study focuses on a disruption mutant of the pehR gene from the local isolate R. solanacearum F1C1, obtained from Tezpur, Assam. Utilizing RNA-seq, we examine the gene expression profiles of R. solanacearum F1C1 under pehR regulation. Differential expression analysis between wild type and pehR mutants reveals a set of genes directly influenced by pehR. This comprehensive gene expression analysis provides insights into the system-level responses orchestrated by the pehR regulator in pathogenicity and virulence development. Moreover, it sheds light on the broader regulatory network controlled by pehR and its underlying molecular mechanisms. Our work also underscores the identification of key target genes regulated by pehR within R. solanacearum F1C1, offering a deeper understanding of its role in bacterial pathogenicity. Ultimately, unraveling the complete pehR regulome holds promise for elucidating the regulatory dynamics driving the pathogenic behavior of R. solanacearum F1C1.

2024-11-22 | GSE269108 | GEO

Ralstonia pseudosolanacearum GMI1000

Project description:Soil-born pathogen that naturally infects plant roots

| PRJNA13 | ENA

ralstonia infection-characterisation of wat1 mutant

Project description:rs08-04_wat1-ralstonia - ralstonia infection - identification of the role of the plant cell wall in the interactions between plants and pathogenic agents - Comparison between the mutant and the wt at different time after infection with ralstonia bacteria Keywords: treated vs untreated comparison

2010-08-01 | GSE19081 | GEO

RNA-seq analysis of Bacteroides xylanisolvens XB1AT grown on pectin or xylan

Project description:Dietary fiber degradation is a key function of the human gut microbiota. The aim of this study was to increase our knowledge on the degradation of plant cell wall polysaccharide degradation by a prominent human gut bacterial species, Bacteroides xylanisolvens. The transcriptome analysis of B. xylanisolvens XB1AT revealed the existence of six and two genomic loci dedicated to the degradation of pectins and xylan, respectively. These loci or PUL ("Polysaccharide Utilization Loci") are known to encode enzyme systems in Bacteroides that are specific to a particular polysaccharide.

2015-10-28 | GSE74379 | GEO

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