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Lotus japonicus strain:Miyakojima MG-20

ABSTRACT: Transcriptome analysis in response to symbiotic nitrogen fixation in Lotus japonicus.

PROVIDER: PRJDB16001 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR483565_1.fastq.gz	Fastqsanger.gz
	DRR483565_2.fastq.gz	Fastqsanger.gz
	DRR483566_1.fastq.gz	Fastqsanger.gz
	DRR483566_2.fastq.gz	Fastqsanger.gz
	DRR483567_1.fastq.gz	Fastqsanger.gz

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Gene expression during root nodule development in wild type and Fix- mutant of Lotus japonicus

Project description:RNA-seq analysis was performed to know the expression profiles of Lotus japonicus genes during nodule development and nitrogen fixation.

2019-12-31 | GSE129778 | GEO

Dataset Lotus Japonicus root exudates

Project description:The present dataset is composed of Lotus japonicus root exudate samples under different nitrogen states: starved (no nitrogen), inorganic N (KNO3), symbiotic N (inoculation with M. loti), and inorganic/symbiotic (KNO3 + M. loti). The samples were analyzed by ultra-high-performance liquid chromatography (UHPLC) coupled to a quadrupole time-of-flight mass spectrometer (qToF MS, Bruker Compact) with electrospray ionization.

2023-05-22 | MSV000092000 | MassIVE

Lotus japonicus strain:Miyakojima MG-20

Project description:Identification of the causative gene of Lotus japonicus late nodulation mutant

| PRJDB5078 | ENA

Cell-type specific transcriptome analysis in nodules of Lotus japonicus

Project description:Legume plants can establish symbiotic nitrogen fixation (SNF) with rhizobia mostly in root nodules, where rhizobia-infected cells are accompanied with uninfected cells in a mosaic pattern. Inside the mature nodules of legume, carbon and nitrogen nutrients between host plant cells and their resident bacteria are actively exchanged. To elucidate the metabolite dynamics relevant for SNF in nodules, three cell-types from nodule tissues of a model legume, Lotus japonicus, were isolated using laser microdissesction, and transcriptome analysis was done by an oligoarray with 60-mer length representing 21,495 genes. In our cell-type-specific profiling, many genes were identified as being expressed in nodules with spatial-specific manners. Among them, genes coding for metabolic enzymes were classified according to their function, and detailed data analysis figured out that secondary metabolic pathway was highly activated in nodule cortex. In particular, a number of metabolic genes for phenyl propanoid pathway were found as highly expressed genes accompanied with those encoding putative transporters of secondary metabolites. These data suggest the involvement of novel physiological function of phenylpropanoids in SNF. Gene expression in three different cell-types of Lotus japonicus nodule was measured. Three independent experiments were performed at each cell-types.

2011-12-29 | E-GEOD-34753 | biostudies-arrayexpress

Lotus japonicus strain:Miyakojima MG-20

Project description:Identification of the causative gene of Lotus japonicus nitrate unresponsive symbiosis 2 mutant

| PRJDB10453 | ENA

Lotus japonicus strain:Miyakojima MG-20

Project description:DNA affinity purification sequencing (DAP-seq) analysis of NRSYM1 transcription factor in Lotus japonicus

| PRJDB10458 | ENA

Transcriptomic changes in Medicago truncatula and Lotus japonicus root nodules during drought stress

Project description:Drought is one of the major environmental factors limiting biomass and seed yield production in agriculture. In this research we focused on plants from Fabaceae family, which have a unique ability for establishment of symbiosis with nitrogen-fixing bacteria, and are relatively susceptible to water limitation. We present the changes in nitrogenase activity and global gene expression occurring in Medicago truncatula and Lotus japonicus root nodules during water deficit. Our results prove a decrease in the efficiency of nitrogen fixation as well as extensive changes in plant and bacterial transcriptomes shortly after watering cessation. We show for the first time that not only symbiotic plant component, but also Sinorhizobium meliloti and Mesorhizobium loti bacteria residing in the root nodules of M. truncatula and L. japonicus, respectively, adjust their gene expression in response to water shortage. Although our results demonstrate that both M. truncatula and L. japonicus root nodules are susceptible to water deprivation, they indicate significant differences in plant and bacterial response to drought between tested species, which may be related to various type of root nodules formed by these species.

2019-02-26 | GSE126986 | GEO

Lotus japonicus strain:Miyakojima MG-20

Project description:Transcriptome analysis in response to overexpression of IRON MAN 1 (LjIMA1) and LjIMA2 genes in Lotus japonicus.

| PRJDB16002 | ENA

Tissue-specific transcriptome analysis in nodules of Lotus japonicus

2011-12-29 | GSE34753 | GEO

Comparative transcriptomic analysis of Lotus japonicus leves and roots under symbiotic and non-symbiotic conditions.

Project description:Microarray transcriptomic analysis was carried out on Lotus japonicus plants grown either under purely symbiotic conditions (Mesorhizobium loti) or under non-symbiotic conditions (no inoculation and provided with NH4NO3).

2020-07-02 | E-MTAB-8235 | biostudies-arrayexpress

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