Plasmodium berghei
Ontology highlight
ABSTRACT:
PROVIDER: PRJDB17148 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DRR518128.fastq.gz | Fastqsanger.gz | |||
| DRR518129.fastq.gz | Fastqsanger.gz | |||
| DRR518130.fastq.gz | Fastqsanger.gz | |||
| DRR518131.fastq.gz | Fastqsanger.gz | |||
| DRR518132.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Malaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in the human and the mosquito hosts regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little is known about its role in Plasmodium. Here we show that Plasmodium berghei expresses a conserved SKP1/Cullin1/FBXO1 complex showing tightly regulated expression and localisation across multiple developmental stages. It regulates cell division by controlling nucleus segregation during schizogony and centrosome partitioning during microgametogenesis. It additionally controls parasite-specific processes including gamete egress from the host erythrocyte, as well as formation of the merozoite apical complex and the ookinete inner membrane complex (IMC), two structures essential for Plasmodium dissemination, moreover it is critical for zygote to ookinete conversation. Ubiquitinomic surveys reveal a large set of proteins ubiquitinated in an FBXO1-dependent manner including proteins important for egress and IMC organisation. We additionally demonstrate bidirectional interplay between ubiquitination and phosphorylation via calcium-dependent protein kinase 1. Altogether we show that Plasmodium SCFFBXO1 plays conserved roles in cell division and additionally controls parasite-specific processes in the mammalian and mosquito hosts.
2023-02-17 | PXD035557 | Pride
Project description:Malaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in the human and the mosquito hosts regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little is known about its role in Plasmodium. Here we show that Plasmodium berghei expresses a conserved SKP1/Cullin1/FBXO1 complex showing tightly regulated expression and localisation across multiple developmental stages. It regulates cell division by controlling nucleus segregation during schizogony and centrosome partitioning during microgametogenesis. It additionally controls parasite-specific processes including gamete egress from the host erythrocyte, as well as formation of the merozoite apical complex and the ookinete inner membrane complex (IMC), two structures essential for Plasmodium dissemination, moreover it is critical for zygote to ookinete conversation. Ubiquitinomic surveys reveal a large set of proteins ubiquitinated in an FBXO1-dependent manner including proteins important for egress and IMC organisation. We additionally demonstrate bidirectional interplay between ubiquitination and phosphorylation via calcium-dependent protein kinase 1. Altogether we show that Plasmodium SCFFBXO1 plays conserved roles in cell division and additionally controls parasite-specific processes in the mammalian and mosquito hosts.
2023-02-17 | PXD035526 | Pride
Project description:Malaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in the human and the mosquito hosts regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little is known about its role in Plasmodium. Here we show that Plasmodium berghei expresses a conserved SKP1/Cullin1/FBXO1 complex showing tightly regulated expression and localisation across multiple developmental stages. It regulates cell division by controlling nucleus segregation during schizogony and centrosome partitioning during microgametogenesis. It additionally controls parasite-specific processes including gamete egress from the host erythrocyte, as well as formation of the merozoite apical complex and the ookinete inner membrane complex (IMC), two structures essential for Plasmodium dissemination, moreover it is critical for zygote to ookinete conversation. Ubiquitinomic surveys reveal a large set of proteins ubiquitinated in an FBXO1-dependent manner including proteins important for egress and IMC organisation. We additionally demonstrate bidirectional interplay between ubiquitination and phosphorylation via calcium-dependent protein kinase 1. Altogether we show that Plasmodium SCFFBXO1 plays conserved roles in cell division and additionally controls parasite-specific processes in the mammalian and mosquito hosts.
2023-04-28 | PXD035539 | Pride
Project description:Malaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in the human and the mosquito hosts regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little is known about its role in Plasmodium. Here we show that Plasmodium berghei expresses a conserved SKP1/Cullin1/FBXO1 complex showing tightly regulated expression and localisation across multiple developmental stages. It regulates cell division by controlling nucleus segregation during schizogony and centrosome partitioning during microgametogenesis. It additionally controls parasite-specific processes including gamete egress from the host erythrocyte, as well as formation of the merozoite apical complex and the ookinete inner membrane complex (IMC), two structures essential for Plasmodium dissemination, moreover it is critical for zygote to ookinete conversation. Ubiquitinomic surveys reveal a large set of proteins ubiquitinated in an FBXO1-dependent manner including proteins important for egress and IMC organisation. We additionally demonstrate bidirectional interplay between ubiquitination and phosphorylation via calcium-dependent protein kinase 1. Altogether we show that Plasmodium SCFFBXO1 plays conserved roles in cell division and additionally controls parasite-specific processes in the mammalian and mosquito hosts.
2023-02-21 | PXD040231 | Pride
Project description:Transcriptional profiling of gametocyte non-producer lines in Plasmodium berghei Transcriptome of gametocyte non producer lines (natural and genetic KO) and parental (820) lines. The aim of the study was to identify key genes involved in the decision to commit to gametocytogenesis in Plasmodium berghei. These microarrays compare naturally selected lines that do not produce gametocytes, and the parental line and additionally a genetic knock out of AP2-G PBANKA_143750. Data published Sinha, Hughes, et, al Nature tbc.
2013-12-13 | GSE53246 | GEO
Project description:Phosphatase type 1 is a major enzyme essential to Plasmodium development. However, the detailed mechanisms underlying its regulation remain to be deciphered. In this work, we report the functional characterization of the Plasmodium berghei LRR1, an ortholog of SDS22, one of the most ancient and conserved Phosphatase type 1 (PP1) interactor. SDS22 has been described as a PP1 regulator essential to critical cellular features such as motility, polarity, epithelium integrity or mitosis completion. Our study shows that in Plasmodium berghei, PbLRR1 is expressed during the intra-erythrocytic developmental cycle and up to the zygote stage during sexual development. PbLRR1 can be found in complex with PbPP1 in both asexual and sexual stages and is able to inhibit the phosphatase activity. Then, genetic analysis demonstrated that PbLRR1 deletion affects the course of the parasite development during early sporogony which manifest by the production of fewer and smaller oocysts. Finally, PbLRR1 interactome analysis associated with phospho-proteomics studies led to the identification of several putative PbLRR1/PbPP1 substrates. Although previously unsuspected PP1 substrates, some of them have been characterized as essential to the parasite sexual development. In addition, and for the first time, we identify the PP1 inhibitor 3 as a substrate of the PP1/LRR1 complex. In summary, this study provides insights into previously unrecognized PbPP1 fine regulation of Plasmodium early sporogony development through its interaction with PbLRR1.
2022-10-14 | PXD030389 | Pride