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Tyzzerella

ABSTRACT: Horizontal gene transfer shapes pathogenic bacteria in multiple sclerosis_bacterial genome

PROVIDER: PRJDB17277 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR524096_subreads.fastq.gz	Fastqsanger.gz
	DRR524097_subreads.fastq.gz	Fastqsanger.gz
	DRR524098_subreads.fastq.gz	Fastqsanger.gz

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human gut metagenome

Project description:Horizontal gene transfer shapes pathogenic bacteria in multiple sclerosis_fecal metagenome

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Project description:Antibiotic resistance is exacerbated by the exchange of antibiotic resistance genes (ARGs) between microbes from diverse habitats. Plasmids are important ARGs mobile elements and are spread by horizontal gene transfer (HGT). In this study, we demonstrated the presence of multi-resistant plasmids from inhalable particulate matter (PM) and its effect on gene horizontal transfer. Three transferable multi-resistant plasmids were identified from PM in a hospital, using conjugative mating assays and nanopore sequencing. pTAir-3 contained 26 horizontal transfer elements and 10 ARGs. Importantly pTAir-5 harbored carbapenem resistance gene (blaOXA) which shows homology to plasmids from human and pig commensal bacteria, thus indicating that PM is a media for antibiotic resistant plasmid spread. In addition, 125 μg/mL PM2.5 and PM10 significantly increased the conjugative transfer rate by 110% and 30%, respectively, and augmented reactive oxygen species (ROS) levels. Underlying mechanisms were revealed by identifying the upregulated expressional levels of genes related to ROS, SOS, cell membranes, pilus generation, and transposition via genome-wide RNA sequencing. The study highlights the airborne spread of multi-resistant plasmids and the impact of inhalable PM on the horizontal transfer of antibiotic resistance.

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