Project description:Insulin resistance drives the development of type 2 diabetes (T2D). In liver, diacylglycerol (DAG) is a key mediator of lipid-induced insulin resistance. DAG activates protein kinase C epsilon (PKCε), which phosphorylates and inhibits the insulin receptor. In rats, a 3-day high fat diet produces hepatic insulin resistance through this mechanism, and knockdown of hepatic PKCε protects against high fat diet-induced hepatic insulin resistance. Here we employ a systems level approach to uncover additional signaling pathways involved in high fat diet-induced hepatic insulin resistance. We used quantitative phosphoproteomics to map global in vivo changes in hepatic protein phosphorylation in chow-fed, high fat-fed, and high fat-fed with PKCε knockdown rats to distinguish the impact of lipid- and PKCε-induced protein phosphorylation.
Project description:Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
Project description:Limosilactobacillus fermentum, a potentially fruit-derived probiotic, improves gut microbiota composition and alleviates cardiometabolic disorders in male rats fed with a high fat diet
Project description:In the animal study, both a decrease of serum alanine aminotransferase level and whole blood gene expression changes were observed in rats fed a MLP-containing high-fat diet compared with rats fed a high-fat diet.
Project description:In the animal study, both a decrease of serum alanine aminotransferase level and whole blood gene expression changes were observed in rats fed a MLP-containing high-fat diet compared with rats fed a high-fat diet.
Project description:Potential mechanism was discovered through expression profiling of a total of 3545 miRNAs in liver of rats fed with high fat diet after HTG treatment. MiRNAs in liver of rats(3 group, normal fat diet group, 3, high fat diet group, 3, high fat diet + HTG treatment, 3) were detected, and differentially expressed miRNAs were analyzed to reveal potential mechanism of HTG in treating dyslipidaemia.
Project description:A total of 49.88 Gb high quality reads of 6 samples (n=3) were obtained. The result of sample distance indicated that the cecal gene expression profiles of rats fed with diets composed of casein and chicken protein were clearly separated into two clusters. The variation of samples in casein protein fed group was higher than that in chicken protein fed group. There were 2524 differential expressed genes between the two groups, of which 871 genes were up regulated in chicken protein diet group compared with casein diet group.
