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Bioreactor metagenome

ABSTRACT: Genome-based Metagenomic Analysis of Candidatus Scalindua with Anammox consortia in Long-term Cultivated Marine Anammox Bacteria Community

PROVIDER: PRJDB17738 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR538836_1.fastq.gz	Fastqsanger.gz
	DRR538836_2.fastq.gz	Fastqsanger.gz
	DRR538837_subreads.fastq.gz	Fastqsanger.gz
	DRR538838_1.fastq.gz	Fastqsanger.gz
	DRR538838_2.fastq.gz	Fastqsanger.gz

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Transcriptome Kuenenia stuttgartiensis bioreactor

Project description:Anaerobic ammonium-oxidising (anammox) bacteria, members of the ‘Candidatus Brocadiaceae’ family, play an important role in the nitrogen cycle and are estimated to be responsible for about half of the oceanic nitrogen loss to the atmosphere. Anammox bacteria combine ammonium with nitrite and produce dinitrogen gas via the intermediates nitric oxide and hydrazine (anammox reaction) while nitrate is formed as a by-product. These reactions take place in a specialized, membrane-bound compartment called the anammoxosome. Therefore, the substrates ammonium, nitrite and product nitrate have to cross the outer-, cytoplasmic- and anammoxosome membranes to enter or exit the anammoxosome. The genomes of all anammox species harbour multiple copies of ammonium-, nitrite- and nitrate transporter genes. Here we investigated how the distinct genes for ammonium-, nitrite- and nitrate- transport were expressed during substrate limitation in membrane bioreactors. Transcriptome analysis of Kuenenia stuttgartiensis planktonic cells under ammonium-limitation showed that three of the seven ammonium transporter genes and one of the six nitrite transporter genes were significantly upregulated, while another ammonium and nitrite transporter gene were downregulated in nitrite limited growth conditions. The two nitrate transporters were expressed to similar levels in both conditions. In addition, genes encoding enzymes involved in the anammox reaction were differentially expressed, with those using nitrite as a substrate being upregulated under nitrite limited growth and those using ammonium as a substrate being upregulated during ammonium limitation. Taken together, these results give a first insight in the potential role of the multiple nutrient transporters in regulating transport of substrates and products in and out of the compartmentalized anammox cell.

2020-06-05 | GSE148825 | GEO

bioreactor metagenome

Project description:Metagenomic analysis of bacteria in a long-term freshwater anammox column reactor

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bioreactor metagenome

Project description:Metagenomic data of anammox consortia fed with nitrous oxide as an additional electron acceptor

| PRJDB16196 | ENA

bioreactor metagenome

Project description:Anammox biomass Metagenomic assembly

| PRJNA286109 | ENA

bioreactor metagenome

Project description:Bioreactor Anammox bacterial community from Nijmegen, The Netherlands - Scalindua species enirchment metagenome

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bioreactor metagenome

Project description:Anammox biomass R2 Metagenomic assembly

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Candidatus Scalindua japonica

Project description:Genome sequence of an marine anammox bacterium, Candidatus "Scalindua japonica"

| PRJDB740 | ENA

bioreactor metagenome

Project description:Metagenomic assemblies from anammox bioreactor sequences

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Candidatus Scalindua

Project description:Candidatus Scalindua sp. overview

| PRJNA266259 | ENA

Proteome of "Candidatus Kuenenia Stuttgartiensis" strain CSTR1 growing planctonically in semi-CSTR reactor

Project description:The dataset provides the whole proteome of the anammox bacterium "Candidatus Kuenenia Stuttgartiensis" strain CSTR1 growing planctonically in semi-CSTR reactor. The bacteria were growing at high growth rate (0.33 d-1) (reactor HRT 3d).

2020-11-11 | PXD018553 | Pride

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