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Enrichment culture

ABSTRACT: Amplicon sequencing analysis of 16S rRNA of partial-nitrification anammox reactor

PROVIDER: PRJDB18291 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR624629_1.fastq.gz	Fastqsanger.gz
	DRR624629_2.fastq.gz	Fastqsanger.gz
	DRR624630_1.fastq.gz	Fastqsanger.gz
	DRR624630_2.fastq.gz	Fastqsanger.gz
	DRR624631_1.fastq.gz	Fastqsanger.gz

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Community composition of bacteria involved in fixed nitrogen loss in the water column of two major oxygen minimum zones in the ocean

Project description:The community composition (in terms of abundance, distribution and contribution of diverse clades) of bacteria involved in nitrogen transformations in the oxygen minimum zones may be related to the rates of fixed N loss in these systems. The abundance of both denirifying and anammox bacteria, and the assemblage composition of denitrifying bacteria were investigated in the Eastern Tropical South Pacific and the Arabian Sea using assays based on molecular markers for the two groups of bacteria. The abundance and distribution of bacteria associated with the fixed N removal processes denitrification and anammox were investigated using quantitative PCR for genes encoding nitrite reductase (nirK and nirS) in denitrifying bacteria and hydrazine oxidase(hzo) and 16S rRNA genesin anammox bacteria. All of these genes had depth distributions with maxima associated with the secondary nitrite maximum in low oxygen waters. NirS was mch more abundant than nirK, and much more abundant than the 16S rRNA gene from anammox bacteria. The ratio of hzo:16S rRNA for anammox was low and variable implying greater unexplored diversity in the the hzo gene. Assemblage composition of the abundant nirS-type denitrifiers was evaluated using a funcitonal gene microarray. Of the nirS archetypes represented on the microarray, very few occurred speficically in one region or depth interval, but the assemblages varied significantly. Community composition of denitrifiers based on microarray analysis of the nirS gene was most different between geographical regions. Within each region, the surface layer and OMZ assemblages clustered distinctly. Thus, in addition to spatial and temporal variation in denitrificaiton and anammox rates, both microbial abundance and community composition also vary between OMZ regions and depths.

2013-09-12 | GSE50787 | GEO

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Project description:One stage partial nitrification/Anammox biofilm reactor Metagenome

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