ABSTRACT: Integration of single nucleus RNA-seq and bulk RNA-seq reveals gene regulatory networks for vascular connection between parasitic plants and host plants
Project description:47,984 unigenes with putative coding regions with at least 50 amino acids were selected from whole transcriptome (SAMN03271814) of Phtheirospermum japonicum Microarray slides were hybridized with labeled cRNA from roots of 2-week-old Phtheirospermum japonicum plants growing axenically and treated with haustoria-inducer DMBQ (10 µM) for 0, 0.5, 1, 3, 6, 12, 24 and 48 h
Project description:Transcriptomic changes during haustoria formation in the parasitic plant Phtheirospermum japonicum. We performed RNAseq on Phtheirospermum japonicum seedling infecting and not infecting over 5 time points during haustoria formation for the control treatment and over 3 time points for the ammonium nitrate and 6-Benzylaminipurine. We investigated the transcriptomic changes during haustoria formation and how this is affected in the transcritpome level by nitrate and cytokinin treatment.
Project description:In this study, we report the complete chloroplast (cp) genome of Phtheirospermum japonicum was determined through Illumina sequencing method. The complete chloroplast genome of Ph. japonicum was 153,397?bp in length and contained a pair of IR regions (25,601?bp) separated by a small single copy region (17,728?bp) and a large single copy region (84,467?bp). The cp genome of Ph. japonicum encoded 125 genes including 86 protein-coding genes, 31 tRNA genes and eight ribosomal RNA genes. The overall GC content of Ph. japonicum cp genome is 38.3%. By phylogenetic analysis using ML method, Ph. japonicum was placed in family Orobanchaceae and showed the closest relationship with Castilleja paramensis.
Project description:The first GSSM of V. vinifera was reconstructed (MODEL2408120001). Tissue-specific models for stem, leaf, and berry of the Cabernet Sauvignon cultivar were generated from the original model, through the integration of RNA-Seq data. These models have been merged into diel multi-tissue models to study the interactions between tissues at light and dark phases.
