Unidentified
Ontology highlight
ABSTRACT:
PROVIDER: PRJDB20035 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comprehensive collection of gene expression profiles for immune cell transcriptome (CBX19)
| PRJDB20009 | ENA
Project description:The self-renewing pluripotent state was first captured in mouse embryonic stem cells (mESCs) over two decades ago. The standard condition requires the presence of serum and LIF, which provide growth promoting signals for cell expansion. However, there are pro-differentiation signals which destabilize the undifferentiated state of mESCs. The dual inhibition (2i) of the pro-differentiation Mek/Erk and Gsk3/Tcf3 pathways in mESCs is sufficient to establish an enhanced pluripotent “ground state” which bears features resembling the pre-implantation mouse epiblast. Gsk3 inhibition alleviates the repression of Esrrb, a transcription factor that can substitute for Nanog function in mESCs. The molecular mechanism that is mediated by Mek inhibition is however not clear. In this study, we investigate the pathway through which Mek inhibition operates to maintain ground state pluripotency. We have found that in mESCs, Kruppel-like factor 2 (Klf2) is a protein target of the Mek/Erk pathway; and that Klf2 protein is phosphorylated by Erk2 and subsequently degraded through the proteosome. It is therefore by Mek-inhibition through PD0325901 or 2i that enables the stabilization and accumulation of Klf2 to sustain ground state pluripotency. Importantly, we found that Klf2-null mESCs, while viable under LIF/Serum conditions, cannot be maintained and eventually gradually die within a few passages. Our result thus demonstrates that Klf2 is an essential factor of ground state pluripotency. Collectively, our study defines the Mek/Klf2 axis that cooperates with the Gsk3/Esrrb pathway in mediating ground state pluripotency.
2014-06-04 | E-MTAB-2365 | biostudies-arrayexpress
Project description:Kanate was used to induce seizures in mice, and gene expression in the hippocampus was measured Using MEK inhibitors, MEK-dependent gene expression was assessed.
2017-06-14 | GSE88723 | GEO
Project description:microRNA expression profiling in renal cell carcinoma. (CBX62)
| PRJDB20039 | ENA
Project description:Maternal and Neonatal Exposure to Environmental Tobacco Smoke Targets Pro-Inflammatory Genes in Neonatal Arteries (CBX26)
| PRJDB20016 | ENA
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| PRJDB20101 | ENA
Project description:Gene expression profile in Jdp2KO MEF cells (CBX101)
| PRJDB20077 | ENA
Project description:Analysis of gene expression levels in BBF2H7-/- chondrocytes. (CBX84)
| PRJDB20062 | ENA