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ABSTRACT:
PROVIDER: PRJDB20085 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparative gene expression analyses using E4.0 cloned blastocysts
| PRJNA213219 | ENA
Project description:Comparison of gene expression between male and female blastocysts
| PRJNA92441 | ENA
Project description:Comparison of gene expression profiles of Prevotella intermedia strain 17 in biofilm and in planktonic condition (CBX27)
| PRJDB20011 | ENA
Project description:Comparison of gene expression profiles between human and mouse monocyte subsets [mouse data]
| PRJNA118939 | ENA
Project description:Whereas cloning mammals by direct somatic cell nuclear transfer has been successful using a wide range of donor cell types, neurons from adult brain remain M-bM-^@M-^\unclonableM-bM-^@M-^] for unknown reasons. Here we examined whether neurons from adult mice could be cloned, using a combination of two epigenetic approaches. First, we used a specific antibody to discover cell types with reduced amounts of a repressive histone mark - dimethylated histone H3 lysine 9 (H3K9me2) - and identified CA1 pyramidal cells in the hippocampus and Purkinje cells in the cerebellum as candidates. Second, reconstructed embryos were treated with trichostatin A (TSA), a potent histone deacetylase inhibitor. Using CA1 cells, cloned offspring were obtained at high rates, reaching 10.2% and 4.6% (per embryos transferred) for male and female donors, respectively. Cerebellar Purkinje cell nuclei were too large to maintain their genetic integrity during nuclear transfer, leading to developmental arrest of embryos. However, gene expression analysis using cloned blastocysts corroborated a high rate of genomic reprogrammability of CA1 pyramidal and Purkinje cells. Neurons from the hippocampal dentate gyrus and cerebral cortex, which had higher amounts of H3K9me2, could also be used for producing cloned offspring, but the efficiencies were low. A more thorough analysis revealed that TSA treatment was essential for cloning adult neuronal cells. This study demonstrated for the first time that adult neurons could be cloned by nuclear transfer. Furthermore, our data imply that reduced amounts of H3K9me2 and increased histone acetylation appear to act synergistically to improve the development of cloned embryos. Comparative gene expression analyses using blastocysts of cloned embryos were performed by microarray. Cloned embryos were produced with three different types of donor cells (neonatal Sertoli cells, CA1 pyramidal cells and Purkinje cells) and all cloned embryos were treated with Trichostatin A (TSA). Each embryos were cultured for 96 h and blastocysts derived from each donor cell types were subjected to gene expression microarray. For comparison of gene expression, the data sets of control sex- and genotype-matched embryos produced by in vitro fertilization and SCNT-derived blastocysts from cumulus cells treated with TSA from our previous paper (Inoue K. et al. Science 2010) were also used.
2015-02-03 | E-GEOD-65497 | biostudies-arrayexpress
Project description:Primary outcome(s): Comparison of the gene expression profile in hair follicle between cancer patients and healthy volunteer
| 2628246 | ecrin-mdr-crc
Project description:Comprehensive collection of gene expression profiles for immune cell transcriptome (CBX19)
| PRJDB20009 | ENA
Project description:Global gene expression profiles in male and female single bovine blastocysts
| PRJNA377331 | ENA
Project description:Genome-Wide Analysis of Abnormal H3K9 Acetylation in Cloned Mice (CBX38)
| PRJDB20023 | ENA
Project description:Comparison of small RNA expression between male and female blastocysts
| PRJDB2145 | ENA