Marine metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJDB20501 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Asterionellopsis glacialis A3 microbial consortium MAGs
| PRJNA588964 | ENA
Project description:White Sea marine sediments prokaryotic MAGs
| PRJNA1048160 | ENA
Project description:Re-assembled SAR202 MAGs from TARA Oceans Metagenomes
| PRJNA547867 | ENA
Project description:GL-1248 core's MAGs from western equatorial Atlantic
| PRJNA637369 | ENA
Project description:Palauibacterales (PAUC43f) MAGs from Mar Menor sediments (Spain)
| PRJNA838580 | ENA
Project description:Metagenome assembled genomes (MAGs) from Gulf of Khambhat pelagic sediments
| PRJNA598413 | ENA
Project description:Metagenome assembled genomes (MAGs) from Gulf of Kutch pelagic sediments
| PRJNA598416 | ENA
Project description:Metagenome-assembled Genomes (MAGs) of multiple cyanobacterial lineages from Oxygen Deficient Marine Zones
| PRJNA1136951 | ENA
Project description:More than 99% of identified prokaryotes, including many from the marine environment, cannot be cultured in the laboratory. This lack of capability restricts our knowledge of microbial genetics and community ecology. Metagenomics, the culture-independent cloning of environmental DNAs that are isolated directly from an environmental sample, has already provided a wealth of information about the uncultured microbial world. It has also facilitated the discovery of novel biocatalysts by allowing researchers to probe directly into a huge diversity of enzymes within natural microbial communities. Recent advances in these studies have led to a great interest in recruiting microbial enzymes for the development of environmentally-friendly industry. Although the metagenomics approach has many limitations, it is expected to provide not only scientific insights but also economic benefits, especially in industry. This review highlights the importance of metagenomics in mining microbial lipases, as an example, by using high-throughput techniques. In addition, we discuss challenges in the metagenomics as an important part of bioinformatics analysis in big data.
| S-EPMC4678775 | biostudies-literature
Project description:Metagenomic analyses of marine viruses generate an overview of viral genes present in a sample, but the percentage of the resulting sequence fragments that can be reassembled is low and the phenotype of the virus from which a given sequence derives is usually unknown. In this study, we employed physical fractionation to characterize the morphological and genomic traits of a subset of uncultivated viruses from a natural marine assemblage. Viruses from Kāne'ohe Bay, Hawai'i were fractionated by equilibrium buoyant density centrifugation in a cesium chloride (CsCl) gradient, and one fraction from the CsCl gradient was then further fractionated by strong anion-exchange chromatography. One of the fractions resulting from this two-dimensional separation appeared to be dominated by only a few virus types based on genome sizes and morphology. Sequences generated from a shotgun clone library of the viruses in this fraction were assembled into significantly more numerous contigs than have been generated with previous metagenomic investigations of whole DNA viral assemblages with comparable sequencing effort. Analysis of the longer contigs (up to 6.5 kb) assembled from our metagenome allowed us to assess gene arrangement in this subset of marine viruses. Our results demonstrate the potential for physical fractionation to facilitate sequence assembly from viral metagenomes and permit linking of morphological and genomic data for uncultivated viruses.
| S-EPMC3620275 | biostudies-literature